EFFECT OF IL-1P ON GROWTH PROPERTIES OF VAGINAL MICROSYMBIONTS

Aim. Study the effect of IL-1 в in concentrations that are characteristic for vaginal normo- and pathocenosis on growth properties ofvaginal microsymbionts. Materials and methods. Concentration ofIL-1 P in vaginal contents ofwomen during bacterial vaginosis and normocenosis was determined by using enzume immunoassay. Changes of growth characteristics and biofilm formation ability of Staphylococcus aureus, Escherichia coli, Lactobacilus spp., Corynebacterium spp. under the effect of various IL-1P concentrations by method of O'Toole G.A. (1999) were studied. Results. IL-1P in concentrations characteristic for normocenosis was shown to be able to cause stimulating effect on growth properties of lactobacilli and corynebacteria and suppress growth of S. aureus and E. coli in both plankton and biofilm cultures. IL-1P concentrations characteristic for vaginal dysbiosis on the contrary result in suppression of growth of lactobacilli biomass against the background ofstimulation ofgrowth properties and biofilm formation ability of S. aureus and E. coli. Conclusion. Differential dose-dependent effect of IL-1P on biomass growth and biofilm formation ability of vaginal microsymbionts is a mechanism of regulation of vaginal microbiocenosis.

IL-1p, bacteria, biofilms, vaginal microbiocenosis, lactobacilli, growth, cytokines