BACTERICIDAL ACTION OF NON-THERMAL PLASMA ON BIOFILMS FORMED IN VITRO AND WITHIN A ROOT CHANNEL


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Abstract

Aim. Evaluation of efficiency of non-thermal plasma as bactericidal agent affecting biofilms formed in vitro and on walls of a root channel. Materials and methods. The multiple antibiotic resistant strain Staphylococcus epidermidis isolated from pulpitis was used. Biofilms formed in vitro on the plastic surface and ex vivo at the walls of the root canal were treated with plasma torch formed by argon:air (9:1) mixture eradiated with 100 kHz electrtomagnetic field. Bacterial viability was determined by plating and by differential Live/Dead labeling. Results. The dose-dependent decrease in living bacteria was demonstrated. The three-step kinetics ofbacterial killing was observed. Total elimination ofup 10 9 CFU/sample was reached at exposition of240 s or more. Conclusion. The non-thermal plasma effectively destroyed bacterial biofilms within root channels.

About the authors

E. V Sysolyatina

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

K. A Sobyanin

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

A. V Petryakov

Troitsk Institute of Innovative and Thermonuclear Research, Moscow, Russia

N. I Trushkin

Troitsk Institute of Innovative and Thermonuclear Research, Moscow, Russia

E. N Beketova

Stomatological policlinic No. 65, Moscow, Russia

O. Yu Arseenkova

Stomatological policlinic No. 65, Moscow, Russia

T. I Karpova

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

A. L Gintsburg

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

Yu. S Akishev

National Research Nuclear University, Moscow, Russia

S. A Ermolaeva

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

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Copyright (c) 2013 Sysolyatina E.V., Sobyanin K.A., Petryakov A.V., Trushkin N.I., Beketova E.N., Arseenkova O.Y., Karpova T.I., Gintsburg A.L., Akishev Y.S., Ermolaeva S.A.

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