MOLECULAR-GENETIC CHARACTERISTICS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM PATIENTS WITH TUBERCULOUS SPONDYLITIS


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Abstract

Aim. Molecular-genetic characteristic of M. tuberculosis strains isolated from operation material of patients with tuberculous spondylitis. Materials and methods. 107 strains of M. tuberculosis isolated in 2007-2011 from patients with spine tuberculosis were studied by methods of spoligotyping and MIRU-VNTR by 12 and 24 loci. Results. Strains of genetic family Beijing dominated (n=80), 78% ofthose had multiple drug resistance (MDR). Strains ofgenetic families T, H3 (Ural), LAM, Manu, H4 and S were also detected. Differentiating of80 strains of Beijing genotype by MIRU-VNTR method by 24 loci revealed 24 variants (HGI=0.83) including 7 clusters, the largest of those (100-32) included 23 strains (87% MDR). Conclusion. The leading role of Beijing genotype M. tuberculosis strains in development of tuberculous spondylitis with multiple drug resistance of the causative agent is shown.

About the authors

A. A Vyazovaya

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

N. S Solovieva

St. Petersburg Research Institute of Phthisiopulmonology, Russia

V. Yu Zhuravlev

St. Petersburg Research Institute of Phthisiopulmonology, Russia

I. V Mokrousov

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

O. A Manicheva

St. Petersburg Research Institute of Phthisiopulmonology, Russia

B. I Vishnevsky

St. Petersburg Research Institute of Phthisiopulmonology, Russia

O. V Narvskaya

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

References

  1. Инфекционная заболеваемость в субъектах Российской Федерации за 2010 - 2011гг. Информационный сборник статистических и аналитических материалов.М., Федеральный центр гигиены и эпидемиологии Роспотребнадзора, 2012.
  2. Картавых А. А., Борисов С. Е., Матвеева М. В. и др. Туберкулез внелегочных локализаций по данным персональных регистров впервые выявленных больных. Туберкулез и болезни легких. 2009,10: 17-26.
  3. Левашев Ю.Н. , Мушкин А.Ю., Гришко А.Н. Внелегочный туберкулез в России: официальная статистика и реальность. Проблемы туберкулеза. 2006,11: 3-6.
  4. Мокроусов И.В. Генетическое разнообразие и эволюция Mycobacterium tuberculosis. Автореф. дисс. д-ра биол. наук, СПб, 2009.
  5. Нарвская О.В. Геномный полиморфизм Mycobacterium tuberculosis и его значение в эпидемическом процессе. Автореф. дисс. д-ра мед. наук, СПб, 2003.
  6. Туберкулез в Российской Федерации, 2010 г. Аналитический обзор статистических показателей, используемых в Российской Федерации. М., 2011.
  7. Balabanova Y, Nikolayevskyy V, Ignatyeva O. et al. Survival of civilian and prisoner drug-sensitive, multi- and extensive drug- resistant tuberculosis cohorts prospectively followed in Russia. PLoS One. 2011, 6 (6): e20531.
  8. Bifani P., Mathema B., Kurepina N. et al. Global dissemination of the Mycobacterium tuberculosis W/Beijing family strains. Trends Microbiol. 2002, 10 (1): 45-52.
  9. Brudey K., Driscoll J., Rigouts L. et al. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol. 2006, 6: 23.
  10. Garg R., Somvanshi D. Spinal tuberculosis: a review. J. Spinal. Cord. Med. 2011, 34 (5): 440454.
  11. Glynn J., Whiteley J., Bifani P. et al. Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis: a systematic review. Emerg. Infect. Dis. 2002, 8 (8): 843-849.
  12. de Steenwinkel J., ten Kate M., de Knegt G. et al. Consequences of noncompliance for therapy efficacy and emergence of resistance in murine tuberculosis caused by the Beijing genotype of Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 2012, 56: 4937-4944.
  13. Kamerbeek J., Schouls L., Kolk A. et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J. Clin. Microbiol. 1997, 35 (4): 907914.
  14. Lasunskaia E., Ribeiro S., Manicheva O. et al. Emerging multi-drug resistant Mycobacterium tuberculosis strains of the Beijing genotype circulating in Russia express a pattern of biological properties associated with enhanced virulence. Microbes. Infect. 2010, 12: 467-475.
  15. Mokrousov I., Narvskaya O., Vyazovaya A. et al. Mycobacterium tuberculosis Beijing genotype in Russia: in search of informative variable-number tandem-repeat loci. J. Clin. Microbiol. 2008, 46 (11): 3576-3584.
  16. Nikolayevskyy V., Gopaul K., Balabanova Y. et al. Differentiation of tuberculosis strains in a population with mainly Beijing-family strains. Emerg. Infect. Dis. 2006, 12 (9): 1406-1413.
  17. Pang Y.,Song Y.,Xia H. et al. Risk factors and clinical phenotypes of Beijing genotype strains in tuberculosis patients in China. BMC Infect. Dis. 2012, 17 (12): 344-354.
  18. Parwati I., van Crevel R., van Soolingen D. Possible underlying mechanisms for successful emergence of the Mycobacterium tuberculosis Beijing genotype strains. Lancet Infect. Dis. 2010, 10 (2): 103 111.
  19. Purwar S., Chaudhari S., Katoch V et al. Determination of drug susceptibility patterns and genotypes of Mycobacterium tuberculosis isolates from Kanpur district, North India. Infect. Genet. Evol. 2011, 11 (2): 469-475.
  20. Rasouli M., Mirkoohi M., Vaccaro A. et al. Spinal tuberculosis: diagnosis and management. Asian Spine J. 2012, 6 (4): 294-308.
  21. Sankar M., Singh J., Angelin Diana S. et al. Molecular characterization of Mycobacterium tuberculosis isolates from North Indian patients with extrapulmonary tuberculosis. Tuberculosis (Edinb). 2012, 6: S1472-9792.
  22. Supply P., Allix C., Lesjean S. et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 2006, 44 (12): 4498-4510.
  23. Van Embden J., Cave M., Crawford J. et al. Strain identification on Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 1993, 31: 406-409.
  24. Van Soolingen D., Qian L., de Haas P.E. et al. Predominance of a single genotype of Mycobacterium tuberculosis in countries of East Asia. J. Clin. Microbiol. 1995, 33 (12): 3234-3238.
  25. Weng C., Ho C., Dou H. et al. Molecular typing of Mycobacterium tuberculosis isolated from adult patients with tubercular spondylitis. J. Microbiol. Immunol. Infect.2013, 46 (1): 19-23.

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Copyright (c) 2013 Vyazovaya A.A., Solovieva N.S., Zhuravlev V.Y., Mokrousov I.V., Manicheva O.A., Vishnevsky B.I., Narvskaya O.V.

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