EFFECT OF PLANT EXTRACTS ON CYTOTOXIC ACTIVITY OF VIBRIO CHOLERAE HEMOLYSIN


Cite item

Full Text

Abstract

Aim. Study of plant extracts that have the ability to neutralize cytotoxic activity of hemolysin. Materials and methods. Preparations of purified and recombinant V. cholerae eltor hemolysin as well as supernatants of V. cholerae strains were used. Determination of cytotoxic activity of hemolysin and neutralizing activity of plant extracts were carried out by using cell cultures СНО-К1 and CaCo2. Results. Out of 9 water extracts only 3 - extracts of Rhei rhizome, Limonium gmeli-nii and Quercus robur neutralized hemolysin in cell culture CHO-K1 and CaCo2, whereas the other extracts - Humulus lupulus, Ocimum basilicum, Chelidonium majus, Juglans regia, Achillea milefolium and Hypericum perforatum did not have anti-cytotoxic effect. Neutralizing properties of extracts are exhibited during their co-incubation with hemolysin preparations and supernatants of V. cholerae strains already within 10 minutes. Conclusion. Plant extracts that have anti-cytotoxic activity against hemolysin are perspective for development of therapeutic-prophylaxis preparations.

About the authors

O. V Markina

Rostov-on-Don Institute of Plague Control, Rostov-on-Don, Russia

L. P Alekseeva

Rostov-on-Don Institute of Plague Control, Rostov-on-Don, Russia

N. V Markin

Research Institute of Biology of Southern Federal University, Rostov-on-Don, Russia

References

  1. Власов В.П., Монахова Е.В., Ушакова И.Е. и др. Гемолизин Vibrio cholerae eltor. Мол. генет., микробиол. вирусол. 1992, 7-8: 14-20.
  2. Телесманич Н.Р., Ломов Ю.М., Подосинникова Л.С. Механизмы реализации гемолитической активности холерных вибрионов. Журн. микробиол. 2007, 4: 85-92.
  3. Телесманич Н.Р., Безуглова Е.В., Мишанькин М.Б. и др. Роль лектина (hlyA) в гемолитической и гемагглютинирующей активности Vibrio cholerae. Журн. микробиол. 2004, 5: 12-16.
  4. Figueroa-Arredondo P., Heuser J.E., Akopyants N. S. et al. Cell vacuolation caused by Vibrio cholerae hemolysin. Infect. Immun. 2001, 69 (3): 1613-1624.
  5. Harris J.R., Palmer M. Cholesterol specificity of some heptameric beta-barrel pore-forming bacterial toxins: structural and functional aspects. Subcell. Biochem. 2010, 51: 579-596.
  6. Kloft N., Neukirch C., Bobkiewicz W. et al. Pro-autophagic signal induction by bacterial pore-forming toxins. Med. Microbiol. Immunol. 2010, 199: 299-309.
  7. Medini F., Ksouri R., Falleh H. et al. Effects of physiological stage and solvent on polyphenol composition, antioxidadant and antimicrobial activities of Limonium densiflorum. J. Med. Plant Research. 2011, 5 (31): 6719-6730.
  8. Morinaga N., Iwamaru Y, Yahiro K. et al. Differential activities of plant polyphenols on the binding and internalization of cholera toxin in Vsro cells. J. Вю1. Chem. 2005, 280 (24): 2330323309.
  9. Norton R., Vucak M., Stalewski H. Vibrio cholerae non-O1 facial cellulitis in a North Queensland, Australian child. Pediatr. Infect. Dis. J. 2001, 20(5): 550-551.
  10. Saha N., Banerjee K. K. Carbohydrate-mediated regulation of interaction of Vibrio cholerae hemolysin with erythrocyte and phospholipid vesicle. J. Biol. Chem. 1997, 272 (1): 162-167.
  11. Saka H.A., Gutierrez M.G., Bocco J.L. et al. The autophagic pathway a cell survival strategy against the bacterial pore-forming toxin Vibrio cholerae cytolysin. Autophagy. 2007, 3(4): 363-365.
  12. Sa'nchez E., GarciaS., Heredia N. Extracts ofedible and medicinal plants damage membranes of Vibrio cholerae. Appl. Environ. Мicrobial. 2010, 76 (20): 6888-6894.
  13. Suankratay C., Phantumchinda K., Tachawiboonsak W. et al. Non-serogroup O:1 Vibrio cholerae bacteremia and cerebritis. Clin. Infect. Dis. 2001, 32 (7): 117-119.
  14. Toda M., Okubo S., Ikigai H. et al. The protective activity of tea against infection by Vibrio cholerae O1. Appl. Bacteriol. 1991, 70 (2): 109-112.
  15. Vidal J.E., Enriquez-Rincon F., Giono-Cerezo S. et al. Culture supernatants from V. chol-erae O1 El Tor strains isolated from different geographic areas induce cell vacuolation and aytotoxicity. Salud. Publica Mex. 2009, 51: 39-47.
  16. Yamamoto K., Al-Omani M., Honda T. et al. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin. Infect. Immun. 1984, 45 (1): 192-196.
  17. Zhang S.J., Zhang SY, Wang L. Studies on polysaccharide of Rheum palmatum L. Zhongguo Zhong Yao Za Zhi. 1993, 18 (11): 679-681.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2013 Markina O.V., Alekseeva L.P., Markin N.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies