Email this article PRODUCTION OF CYTOKINES BY VAGINAL EPITHELIOCYTES IN THE PROCESS OF INTERACTION WITH DOMINANT AND ASSOCIATIVE MICROSYMBIONTS
- Authors: Kremleva EA1, Cherkasov SV1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 114-118
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2012
- URL: https://microbiol.crie.ru/jour/article/view/13777
- ID: 13777
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Abstract
Aim. Study the expression of cytokines by vaginal epitheliocytes in the process of interaction
with dominant and associative microsymbionts. Materials and methods. IL-8, IL-6, IL-1 and
TNF expression in response to interaction with heat inactivated Lactobacillus spp., Staphylococcus
aureus, Escherichia coli, Corynebacterium spp. or their secretory products in comparison with
basal expression of cytokines by vaginal epitheliocytes was studied. Results. Lactobacilli secretory
products were shown not to influence the expression of IL-8 and IL-1 and moderately stimulated
IL-6 and TNF expression. Contact of epitheliocytes with heat inactivated lactobacilli increased
secretion of IL-8, IL-6 and IL-1 and reduced TNF production. Secretory products of
S. aureus and E. coli caused stimulation of IL-6, IL-1 production and practically did not change
the expression of IL-8 and TNF. Contact of epitheliocytes with heat inactivated S. aureus suppressed
TNF production and had no influence on IL-8, IL-6 and IL-1 expression, contact with
E. coli stimulated TNF and IL-1 expression and suppressed IL-6 expression. Changes in cytokine
expression during interaction of epitheliocytes with corynebacteria were largely similar to the
results of interaction with lactobacilli except IL-6 production that was markedly stimulated by
corynebacteria secretory products. Conclusion. In epithelial-bacterial interactions dominant
and associative microorganisms have a differential effect on functional status of mucosal epitheliocytes
manifesting in production of cytokines that could be the basis of mucosal immunity
regulation.
with dominant and associative microsymbionts. Materials and methods. IL-8, IL-6, IL-1 and
TNF expression in response to interaction with heat inactivated Lactobacillus spp., Staphylococcus
aureus, Escherichia coli, Corynebacterium spp. or their secretory products in comparison with
basal expression of cytokines by vaginal epitheliocytes was studied. Results. Lactobacilli secretory
products were shown not to influence the expression of IL-8 and IL-1 and moderately stimulated
IL-6 and TNF expression. Contact of epitheliocytes with heat inactivated lactobacilli increased
secretion of IL-8, IL-6 and IL-1 and reduced TNF production. Secretory products of
S. aureus and E. coli caused stimulation of IL-6, IL-1 production and practically did not change
the expression of IL-8 and TNF. Contact of epitheliocytes with heat inactivated S. aureus suppressed
TNF production and had no influence on IL-8, IL-6 and IL-1 expression, contact with
E. coli stimulated TNF and IL-1 expression and suppressed IL-6 expression. Changes in cytokine
expression during interaction of epitheliocytes with corynebacteria were largely similar to the
results of interaction with lactobacilli except IL-6 production that was markedly stimulated by
corynebacteria secretory products. Conclusion. In epithelial-bacterial interactions dominant
and associative microorganisms have a differential effect on functional status of mucosal epitheliocytes
manifesting in production of cytokines that could be the basis of mucosal immunity
regulation.
References
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