Email this article EFFECT OF SULFATED POLYSACCHARIDES FROM BROWN ALGAE ON PROLIFERATIVE ANDCYTOTOXIC ACTIVITY OF MICE SPLENOCYTES
- Authors: Makarenkova ID1, Semenova IB1, Akhmatova NK1, Besednova NN1, Zvyagintseva TN1, Ermakova SP1, Makarenkova ID2, Semenova IB2, Akhmatova NK1, Besednova NN3, Zvyagintseva TN4, Ermakova SP4
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Affiliations:
- Mechnikov Research Institute of Vaccines andSera, Moscow
- Research Institute of Epidemiology and Microbiology, Vladivostok
- Pacific Institute of Bioorganic Chemistry, Vladivostok
- Issue: Vol 89, No 6 (2012)
- Pages: 68-75
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2012
- URL: https://microbiol.crie.ru/jour/article/view/13737
- ID: 13737
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Abstract
Materials and methods. Proliferative and cytotoxic activity of mice splenocytes in vitro and ex vivo were studied
in lymphocyte blast transformation reaction and in cytotoxic MTT-test on K562 human erythroblast
leucosis cell line. Microphotography and microscopy were performed by using Axiocam HS photosystem
and computer program AxioVision 4 (Germany). Results. Fucoidans from brown algae Fucus evanescens,
Laminaria cichorioides and Laminaria japonica in vitro and ex vivo systems were established to increase proliferative
activity of mice splenocytes which is evidenced by an increase of stimulation index. Results of in
vitro and ex vivo cytotoxic activity studies demonstrate that fucoidans which are various by chemical structure
stimulate activity of NK-cells and facilitate an increase of splenocyte cytotoxic potential level against NK
sensitive K562 cell line. Conclusion. The data obtained from the study demonstrate an ability of fucoidans to
stimulate splenocyte proliferation and NK-cell killer activity, and studies of relation between structure and
functions of sulfated polysaccharides facilitate a more detailed understanding of aspects of their mechanism
of action on innate immunity system, thus providing the basis for development of new immunobiologic
preparations - modifiers/agonists of innate immunity.
About the authors
I D Makarenkova
I B Semenova
N K Akhmatova
N N Besednova
T N Zvyagintseva
S P Ermakova
I D Makarenkova
Mechnikov Research Institute of Vaccines andSera, MoscowMechnikov Research Institute of Vaccines andSera, Moscow
I B Semenova
Mechnikov Research Institute of Vaccines andSera, MoscowMechnikov Research Institute of Vaccines andSera, Moscow
N K Akhmatova
N N Besednova
Research Institute of Epidemiology and Microbiology, VladivostokResearch Institute of Epidemiology and Microbiology, Vladivostok
T N Zvyagintseva
Pacific Institute of Bioorganic Chemistry, VladivostokPacific Institute of Bioorganic Chemistry, Vladivostok
S P Ermakova
Pacific Institute of Bioorganic Chemistry, VladivostokPacific Institute of Bioorganic Chemistry, Vladivostok
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