Email this article ANALYSIS OF BIOLOGICAL PROPERTIES OF STRAINS-PRODUCERS OF A NUMBER OFPSEUDOMONAS AERUGINOSA RECOMBINANT PROTEINS
- Authors: Soldatenkova AV1, Leonova EI1, Zlygostev SA1, Mikhaylova NA1, Soldatenkova AV2, Leonova EI2, Zlygostev SA2, Mikhaylova NA2
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
- Issue: Vol 89, No 6 (2012)
- Pages: 60-64
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13727
- ID: 13727
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Abstract
proteins compared with the initial strains. Materials and methods. 5 Escherichia coli strains (initial
strains E. сoli M15 and E. сoli BL21(DE3) and strains-producers of OprL, OprF, aTox3 recombinant proteins
(E. coli М15/oprF, E. coli М15/oprL, E. coli BL21-аTox3) were studied. Toxicity, toxigenicity and virulence
determination were carried out in experiments in non-linear mice. Enzymatic properties of the initial strains
and strains-producers were compared in a number of biochemical tests. Results. The studied strains-producers
of the most immunogenic P. aeruginosa recombinant proteins were confirmed to be biosafe, belong to
Enterobacteriaceae family and Escherichia genus. As a result of genetic engineering manipulations carried out
with E. coli М15 and E. coli BL21(DE3) alterations of biochemical and growth properties, virulence, to xicity
and toxigenicity in the constructed strains-producers of recombinant proteins - E. coli М15/oprF, E. coli
М15/oprL, E. coli BL21-аTox3 - were not detected. Conclusion. The results obtained allow to consider the
possibility of use of E. coli М15/oprF, E. coli М15/oprL and E. coli BL21-аTox3 strains-producers for production
and isolation of candidate proteins for inclusion into vaccine against pseudomonas infection.
About the authors
A V Soldatenkova
E I Leonova
S A Zlygostev
N A Mikhaylova
A V Soldatenkova
Mechnikov Research Institute of Vaccines and Sera, Moscow, RussiaMechnikov Research Institute of Vaccines and Sera, Moscow, Russia
E I Leonova
Mechnikov Research Institute of Vaccines and Sera, Moscow, RussiaMechnikov Research Institute of Vaccines and Sera, Moscow, Russia
S A Zlygostev
Mechnikov Research Institute of Vaccines and Sera, Moscow, RussiaMechnikov Research Institute of Vaccines and Sera, Moscow, Russia
N A Mikhaylova
Mechnikov Research Institute of Vaccines and Sera, Moscow, RussiaMechnikov Research Institute of Vaccines and Sera, Moscow, Russia
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