CHANGES IN OXIDATIVE STRESS AND APOPTOSISPARAMETERS OF NEUTROPHILS INRHEUMATOID DISEASES

Aim. Study parameters of oxidative stress and
apoptosis of neutrophils in patients with ankylosing
spondylitis, systemic lupus erythematosus,
systemic scleroderma and rheumatoid heart
disease. Materials and methods. 240 patients with
rheumatoid diseases and 25 healthy control
group volunteers were examined. Neutrophil
isolation from peripheral blood was performed
by using double density gradient of ficoll-urografin.
Cell functional activity was studied by
chemiluminescence method. Pro-apoptosis
antigen bak expression by neutrophils was studied
by using streptavidin-biotin method. Griss
reagent was used for nitrogen oxide production
analysis. Results. An increase of oxygen dependent
neutrophil metabolism processes was detected
in patients with ankylosing spondylitis in
comparison with systemic lupus erythematosus
and systemic scleroderma patients. Systemic
lupus erythematosus is characterized by higher
biocidity of neutrophils in comparison with
systemic scleroderma and rheumatoid heart
disease. Increase of neutrophil granulocyte
activity in ankylosing spondylitis is accompanied
by an increase of superoxide-anion formation.
In spite of high level of metabolic activity neutrophils
in patients with ankylosing spondylitis
have a low functional reserve, and neutrophils
in patients with systemic scleroderma have the
highest reserve potential. Conclusion. Differences
in expression parameters of oxidative stress by
neutrophils depend on nosological form, varying
by production level and active oxygen form
formation reserve.

neutrophils, oxidative stress, apoptosis