Email this article INFLUENCE OF CORYNEBACTERIA METABOLITES ON ANTAGONISTIC ACTIVITY OF H2O2 PRODUCING LACTOBACILLI
- Authors: Bukharin OV1, Sgibnev AV1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 48-51
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13706
- ID: 13706
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Abstract
Aim. Study combined influence of Corynebacterium genus bacteria metabolites and H2O2 producing
lactobacilli on survival rate of Staphylococcus aureus, Escherichia coli and Lactobacillus
acidophilus. Materials and methods. The ability to inhibit catalase of the test strains used and to
reduce bactericidal effect of hydroxyl radical were determined in corynebacteria. H2O2 containing
metabolites were obtained by cultivating lactobacilli in mineral medium, the amount of H2O2 was
determined by oxidation of TMB by peroxidase. Bactericidal effect of lactobacilli metabolites for
test strains treated by corynebacteria metabolites was evaluated by seeding results. Results. Inhibition
by corynebacteria metabolites of S. aureus catalase activity by 30 - 40% and E. coli catalase activity
by 40 - 70% was shown. A reduction of bactericidal effect of hydroxyl radicals by corynebacteria
metabolites by 30 - 35% for S. aureus, 38 - 42% for E. coli and 70 - 73% for L. acidophilus
was noted. The enchantment of bactericidal effect of lactobacilli after treatment of the test strains
by corynebacteria metabolites against S. aureus and E. coli manifested by reduction of the number
of viable cells by 2 - 3 lg CFU. For L. acidophilus the bactericidal effect of lactobacilli metabolites
in the same conditions reduced, and that led to the increase of viability by 2 - 4 lg PFU. Conclusion.
A conclusion on the possibility of regulation by associative bacteria the manifestations of antagonistic
activity of H2O2 producing dominant microorganisms is made based on the data obtained.
lactobacilli on survival rate of Staphylococcus aureus, Escherichia coli and Lactobacillus
acidophilus. Materials and methods. The ability to inhibit catalase of the test strains used and to
reduce bactericidal effect of hydroxyl radical were determined in corynebacteria. H2O2 containing
metabolites were obtained by cultivating lactobacilli in mineral medium, the amount of H2O2 was
determined by oxidation of TMB by peroxidase. Bactericidal effect of lactobacilli metabolites for
test strains treated by corynebacteria metabolites was evaluated by seeding results. Results. Inhibition
by corynebacteria metabolites of S. aureus catalase activity by 30 - 40% and E. coli catalase activity
by 40 - 70% was shown. A reduction of bactericidal effect of hydroxyl radicals by corynebacteria
metabolites by 30 - 35% for S. aureus, 38 - 42% for E. coli and 70 - 73% for L. acidophilus
was noted. The enchantment of bactericidal effect of lactobacilli after treatment of the test strains
by corynebacteria metabolites against S. aureus and E. coli manifested by reduction of the number
of viable cells by 2 - 3 lg CFU. For L. acidophilus the bactericidal effect of lactobacilli metabolites
in the same conditions reduced, and that led to the increase of viability by 2 - 4 lg PFU. Conclusion.
A conclusion on the possibility of regulation by associative bacteria the manifestations of antagonistic
activity of H2O2 producing dominant microorganisms is made based on the data obtained.
References
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