GENETIC CHARACTERISTIC OF VIRULENT POTENTIAL OF ENTEROCOCCI OF HUMAN INTESTINE MICROBIOTA


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Abstract

Aim. Determine prevalence of genetic determinants of virulence among enterococci strains
comprising human intestine microbiota. Materials and methods. 81 enterococci strains isolated
from intestine of individuals during examination for dysbiosis were used in the study. Strain identification
was performed by using multiplex PCR. Hemolytic and gelatinase activity was determined
by dish method; genes coding virulence factor synthesis (gelE, sprE, cylM, cylB, cylA, esp) - by
PCR. Results. A wide set of genetic determinants of virulence was detected in E. faecalis strain
microorganisms. Conclusion. Enterococcus genus microorganisms of human intestine microbiota
that have virulence potential may become the reason for endogenous infection. The data obtained
may be used for prognosis of risk of development of endogenous enterococci infections.

About the authors

I V Valysheva

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