Email this article STUDY OF EXPRESSION OF CYTOKINEGENES IN THE PROCESS OF CULTIVATIONOF HEALTHY DONOR LEUKOCYTES
- Authors: Koval'chuk LV1, Gankovskaya LV1, Mezentseva MV1, Shapoval IM1, Aitova Y.V1, Magomedova AM1, Romanovskaya VV1, Kovalchuk LV2, Gankovskaya LV2, Mezentseva MV3, Shapoval IM3, Aitova Y.V2, Magomedova AM3, Romanovskaya VV3
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Affiliations:
- Pirogov Russian National Research MedicalUniversity, Moscow,Russia
- Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
- Issue: Vol 89, No 2 (2012)
- Pages: 60-63
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13695
- ID: 13695
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Abstract
in the process of cultivation of healthy donor
leukocytes. Materials and methods. RNA isolated
before, after 3 and 24 hours of cultivation
of leukocytes of 15 healthy donors aged 19 - 32
years was the object of the study. Study of the
expression of genes of 8 cytokines by the level
of their mRNA was performed by using polymerase
chain reaction with reverse transcription.
Results. In healthy donors among with individual
differences, general regularities were
traced: higher levels of IFN and anti-inflammatory
cytokine gene expression, as well as
predominantly low expression of pro-inflammatory
cytokines. Conclusion. In most of the
cases in the process of cultivation of leukocytes
isolated from blood donors the highest level of
gene expression of pro-inflammatory cytokines
is noted after 3 hours of cultivation, and expression
of genes of anti-inflammatory cytokines
remains constant for 24 hours of cultivation.
Keywords
About the authors
L V Koval'chuk
L V Gankovskaya
M V Mezentseva
I M Shapoval
Yu V Aitova
A M Magomedova
V V Romanovskaya
L V Kovalchuk
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
L V Gankovskaya
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
M V Mezentseva
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
I M Shapoval
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
Yu V Aitova
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
A M Magomedova
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
V V Romanovskaya
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
References
- Ковальчук Л.В. Ганковская Л.В., Мешкова Р.Я. Клиническая иммунология и аллергология с основами общей иммунологии. М., Гэотар-Медиа, 2011.
- Ковальчук Л.В., Ганковская Л.В., Рубакова Э.И. Система цитокинов. М., Янус-К, 2000.
- Ковальчук Л.В., Игнатьева Г.А., Ганковская Л.В. Иммунология. Практикум. Клеточные, молекулярные и генетические методы исследования. М., Гэотар-Медиа, 2011.
- Мезенцева М.В. Закономерности функционирования и направленная коррекция цитокиновой регуляторной сети.Дисс. д-ра биол. наук. М., 2006.
- Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinum thyocyoanate-phenol-chloroform extraction. Anal. Biochem. 1987. 162 (1): 156-159.
- Gelder С.М., Thomas P.S., Yates D.H. et al. Cytokine expression in normal, atopic, and asthmatic subject using the combination of sputum induction and the polymerase chain reaction. Thorax. 1995. 50 (10): 1033-1037.
- Holloway A.F., Rao S., Shannon M.F. Regulation of cytokine gene transcription in the immune system. Mol. Immunol. 2002. 38 (8):567-580.
- Paradowska-Gorycka A. Interleukin-10 gene promoter polymorphism in Polish rheumatoid. Intern. J. Immunogenetics. 2010. 37: 225-226.
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