Email this article STUDY OF EXPRESSION OF CYTOKINEGENES IN THE PROCESS OF CULTIVATIONOF HEALTHY DONOR LEUKOCYTES
- Authors: Koval'chuk LV1, Gankovskaya LV1, Mezentseva MV1, Shapoval IM1, Aitova Y.V1, Magomedova AM1, Romanovskaya VV1, Kovalchuk LV2, Gankovskaya LV2, Mezentseva MV3, Shapoval IM3, Aitova Y.V2, Magomedova AM3, Romanovskaya VV3
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Affiliations:
- Pirogov Russian National Research MedicalUniversity, Moscow,Russia
- Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
- Issue: Vol 89, No 2 (2012)
- Pages: 60-63
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.06.2012
- URL: https://microbiol.crie.ru/jour/article/view/13695
- ID: 13695
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Abstract
in the process of cultivation of healthy donor
leukocytes. Materials and methods. RNA isolated
before, after 3 and 24 hours of cultivation
of leukocytes of 15 healthy donors aged 19 - 32
years was the object of the study. Study of the
expression of genes of 8 cytokines by the level
of their mRNA was performed by using polymerase
chain reaction with reverse transcription.
Results. In healthy donors among with individual
differences, general regularities were
traced: higher levels of IFN and anti-inflammatory
cytokine gene expression, as well as
predominantly low expression of pro-inflammatory
cytokines. Conclusion. In most of the
cases in the process of cultivation of leukocytes
isolated from blood donors the highest level of
gene expression of pro-inflammatory cytokines
is noted after 3 hours of cultivation, and expression
of genes of anti-inflammatory cytokines
remains constant for 24 hours of cultivation.
Keywords
About the authors
L V Koval'chuk
L V Gankovskaya
M V Mezentseva
I M Shapoval
Yu V Aitova
A M Magomedova
V V Romanovskaya
L V Kovalchuk
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
L V Gankovskaya
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
M V Mezentseva
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
I M Shapoval
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
Yu V Aitova
Pirogov Russian National Research MedicalUniversity, Moscow,RussiaPirogov Russian National Research MedicalUniversity, Moscow,Russia
A M Magomedova
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
V V Romanovskaya
Gamaleya Research Institute ofEpidemiology and Microbiology, Moscow,RussiaGamaleya Research Institute ofEpidemiology and Microbiology, Moscow,Russia
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