Email this article EFFECT OF CHOLEROGEN AND PROTAMINE ON TIGHT JUNCTIONS OF RAT ENTEROCYTESAND COLONOCYTES
- Authors: Rybal'chenko OV1, Bondarenko VM1, Fal'chuk EL1, Lebed'kova Y.A1, Markov AG1, Rybalchenko OV2, Bondarenko VM3, Falchuk EL2, Lebedkova Y.A2, Markov AG2
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Affiliations:
- St. Petersburg State University, Russia
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
- Issue: Vol 89, No 6 (2012)
- Pages: 3-7
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13689
- ID: 13689
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Abstract
under the effect of cholerogen and protamine. Materials and methods. Cholerogen (cholera toxin,
Sigma-Aldrich, Germany) and protamine sulfate (Russia) were used in the study. The study was carried out
in Wistar line rats. Effect of cholera toxin and protamine on rat intestine epitheliocytes was carried out by
incubating intestine segments in the respective solutions. Ultrastructure changes caused by cholerogen and
protamine in rat enterocytes and colonocytes were assessed based on ultrathin section analysis by transmission
electron microscopy of the cells themselves and tight junctions between them compared with control.
Results. Effect of cholerogen on intestine mucous membrane epitheliocytes manifested in changes of cell
ultrastructure, the form of which transformed as a result of increase of intercellular space without the destruction
of tight junctions. Disappearance of cell plasma membrane lateral area folding and decrease of number
of microvilli was noted. Enlargement of nuclei was noted only in individual cells. Effect of protamine on
epithelial cell layer ultrastructure differed significantly from the effect of cholerogen. Increase of cell plasma
membrane lateral area folding and significant enlargement of nuclei that moved to the central part of cells
reaching its apical end were characteristic effects for protamine. Surface of a part of epitheliocytes lost microvilli with simultaneous destruction of tight junction structure. Protamine induced increase of folding
only in colon without affecting jejunum. At the same time both of these substances caused increase of intercellular
space in jejenum and colon epithelium. Conclusion. Differences in ultrathin structure of rat small
intestine and colon epitheliocyte tight junctions under the effect of cholerogen and protamine were revealed.
About the authors
O V Rybal'chenko
V M Bondarenko
E L Fal'chuk
Yu A Lebed'kova
A G Markov
O V Rybalchenko
St. Petersburg State University, RussiaSt. Petersburg State University, Russia
V M Bondarenko
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, RussiaGamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
E L Falchuk
St. Petersburg State University, RussiaSt. Petersburg State University, Russia
Yu A Lebedkova
St. Petersburg State University, RussiaSt. Petersburg State University, Russia
A G Markov
St. Petersburg State University, RussiaSt. Petersburg State University, Russia
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