MORPHOMETRIC CHARACTERISTICS OFTULAREMIA INFECTIOUS PROCESS IN THEORGANISM OF IMMUNIZED GUINEA PIGS

Aim. Morphometric characteristic of organ
and system state of guinea pigs immunized with
live tularemia vaccine during infection with
virulent culture of tularemia. Materials and
methods. Morphometric study of histological
material from immunized guinea pigs infected
subcutaneously at day 30 with a culture of virulent
tularemia strain was performed. A standard
scheme of sampling and preparation of morphological
material and staining of final semifine
section with hematoxylin and eosin, impregnation
with silver by Masson in Gamperl and
Grimelius modification was used. Morphometric
study was performed by using «Densitomorphometry
» program. Results. Morphometric
parameters that characterize functional state of
organs and systems in immunized, immunized
with consequent infection and infected guinea
pigs were established. Reactive processes that
take place in the infected animal organism
against the background of prior immunization
fit into the range of adaptation-compensation
reactions. Conclusion. The morphometric study
carried out allowed to adequately evaluate the
state of functionally important systems of the
organism of experimental animals, this allows
to consider perspective the wider use of morphometric
analysis in experimental morphology.

infection, live tularemia vaccine, morphometric study