Email this article CHARACTERISTICS OF BORDETELLA PERTUSSISSTRAINS ISOLATED FROM PERTUSSISPATIENTS IN MOSCOW BY USING MULTILOCUSSEQUENCING
- Authors: Borisova OY.1, Mazurova IK1, Ivashinnikova GA1, Gadua NT1, Rudakova IA1, Salova NY.1, Trebunskikh IP1, Skachkova VG1, Panferova RA1, Mooi F1, Aleshkin VA1, Borisova OY.2, Mazurova IK3, Ivashinnikova GA3, Gadua NT3, Rudakova IA3, Salova NY.4, Trebunskikh IP5, Skachkova VG6, Panferova RA6, Mooi F7, Aleshkin VA2
-
Affiliations:
- Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology, Russia
- Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,Russia
- Centre of Hygiene andEpidemiology in Moscow,Russia
- Centre of Hygiene andEpidemiology in Moscow, Russia
- Branch of Centreof Hygiene and Epidemiology in North-WesternAdministrative Region of Moscow, Russia
- Centre forInfectios Diseases Control, National Institutefor Public Health and the Environment, Bilthoven,Netherlands
- Issue: Vol 89, No 2 (2012)
- Pages: 28-34
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13663
- ID: 13663
Cite item
Full Text
Abstract
from pertussis patients in Moscow.
Materials and methods. 53 strains of B. pertussis
isolated from pertussis patients in Moscow in
2007 - 2010 as well as 3 vaccine strains currently
used in Russia for the production of DTP
vaccine were studied by multilocus sequencing
(MLST) based on allele combinations of ptxA,
ptxC and tcfA genes. Results. A genetic characteristic
of B. pertussis strains isolated from
pertussis patients in Moscow by using MLST is
presented. Allele profile analysis of the studied
B. pertussis strains was performed, 4 sequence
types (ST) were identified - ST1, ST2, ST3 and
ST5, most of the circulating strains (86.7%) were
shown to belong to ST5, equal percentage of
cases (5.7%) - to ST2 and ST3, and 1.9% - to
ST1, while 2 vaccine production strains belong
to ST2 and 1 - to ST1. Conclusion. Clonal
structure of contemporary Moscow strains was
shown to be different from strain structure used
for the production of DTP vaccine.
About the authors
O Yu Borisova
I K Mazurova
G A Ivashinnikova
N T Gadua
I A Rudakova
N Ya Salova
I P Trebunskikh
V G Skachkova
R A Panferova
F Mooi
V A Aleshkin
O Yu Borisova
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology, RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology, Russia
I K Mazurova
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,Russia
G A Ivashinnikova
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,Russia
N T Gadua
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,Russia
I A Rudakova
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology,Russia
N Ya Salova
Centre of Hygiene andEpidemiology in Moscow,RussiaCentre of Hygiene andEpidemiology in Moscow,Russia
I P Trebunskikh
Centre of Hygiene andEpidemiology in Moscow, RussiaCentre of Hygiene andEpidemiology in Moscow, Russia
V G Skachkova
Branch of Centreof Hygiene and Epidemiology in North-WesternAdministrative Region of Moscow, RussiaBranch of Centreof Hygiene and Epidemiology in North-WesternAdministrative Region of Moscow, Russia
R A Panferova
Branch of Centreof Hygiene and Epidemiology in North-WesternAdministrative Region of Moscow, RussiaBranch of Centreof Hygiene and Epidemiology in North-WesternAdministrative Region of Moscow, Russia
F Mooi
Centre forInfectios Diseases Control, National Institutefor Public Health and the Environment, Bilthoven,NetherlandsCentre forInfectios Diseases Control, National Institutefor Public Health and the Environment, Bilthoven,Netherlands
V A Aleshkin
Gabrichevsky Moscow Research Institute ofEpidemiology and Microbiology, RussiaGabrichevsky Moscow Research Institute ofEpidemiology and Microbiology, Russia
References
- Борисова О.Ю., Петрова М.С., Мазурова И.К. и др. Особенности коклюшной инфекции в различные периоды эпидемического процесса в г. Москве. Журн. эпидемиол. вакцинопроф. 2010, 4: 33-39.
- Борисова О.Ю., Мазурова И.К., Гадуа Н.Т. и др. Молекулярно-генетические особенности структуры гена, кодирующего фактор колонизации трахеи штаммов B.pertussis. Журн. микробиол. 2010, 5: 11-15.
- Мазурова И.К., Борисова О.Ю., Комбарова С.Ю. и др. Молекулярно-гентическая характеристика штаммов Bordetella pertussis, выделенных в различные периоды эпидемического процесса коклюшной инфекции. Журн. мол. ген. микробиол. вирусол. 2005, 4: 21-25.
- Мазурова И.К., Борисова О.Ю., Комбарова С.Ю. и др. Динамика изменчивости основных генов патогенности штаммов Bordetella pertussis, выделенных от больных коклюшем в г. Москве (1948-2005 гг.). Журн. мол. мед. 2008, 1: 40-45.
- Петрова М.С., Сигаева Л.А., Попова О.П. и др. Особенности эпидемиологии и клиники коклюша в период эпидемического неблагополучия. В: Проблемы инфекционных болезней. 2000, 1, с. 80-86.
- Семенов Б.Ф., Захарова Н.С., Мазурова И.К. Подъем заболеваемости коклюшем на фоне массовой вакцинации. Гипотезы, объясняющие этот феномен. Журн. микробиол. 2003, 6: 70-73.
- Чистякова Г.Г., Борисова О.Ю., Лыткина И.Н. и др. Особенности эпидемического процесса коклюшной инфекции в Москве на современном этапе. Журн. микробиол. 2005, 5: 35-40.
- Шинкарев А.С, Мерцалова Н.У., Мазурова И.К. и др. Современные штаммы Bordetella pertussis: молекулярная и иммунобиологическая характеристика. Журн. микробиол. 2007, 4: 20-25.
- Borisova O., Kombarova S.Y., Zakharova N.S. et al. Antigenic divergence between B. pertussis clinical isolates from Moscow, Russia, and vaccine strains. J. Clin. Vac. Immunol. 2007, 14 (28): 234-238.
- Kourova N., Njamkepo E., Brun D. et al. Monitoring of Bordetella isolates circu lating in Saint Petersburg, Russia between 2001 and 2009. Res. Microbiol. 2010, 161 (10): 810-815.
- Litt D.J., Neal S.E., Fry N.K. Changes in genetic diversity of the Bordetella parapertussis population in the United Kingdom between 1920 and 2006 reflect vaccination coverage and emergence of a single do minant clonal type. J. Clin. Microbiol. 2009, 47 (3): 680-688.
- Mooi F.R. Bordetella pertussis and vaccination: The persistence of a genetically monomorphic pathogen. J. Infect. Genet. Evolut. 2009. 2: 1-14.
- Packard E. R., Parton R., Coote J. et al. Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK. J. Med. Microbiol. 2004, 53:355-365.
- van Amersfoorth S. C. M., Schouls L. M., van der Heide H.G.J. et al. Analysis of Bordetella pertussis populations in european countries with different vaccination policies. J. Clin. Microbiol. 2005, 43: 2837-2843.
- van Loo I. H. M, Heuvelman K. J., King A. J. et all. Multilocus sequence typing of Bordetella pertussis based on surface protein genes. J. Clin Microbiol. 2002, 40: 1994-2001.
Supplementary files
