STUDY OF THE STATE OF PARIETAL MICROFLORAAND WALL OF THE LARGE INTESTINEOF MICE UNDER THE INFLUENCE OFANOMALOUS MAGNETIC FIELD

Aim. Study the possible qualitative and quantitative
changes of microbial community of the
parietal mucin of the large intestine and the state
of the wall of the large intestine in experimental
animals under background and anomalous influence
of geomagnetic field. Materials and methods.
CBA mice were put under the influence
of anomalous magnetic field comparable to its
intensity in Zheleznogorsk (3 Oe) for 1 and 2
weeks. Quantitative and qualitative study of
mucous microflora of the large intestine of the
mice was performed by bacteriological method.
Identification of the microorganisms was performed
by microbiological analyzer «Multiskan-
Ascent» and commercial test-systems «Lachema-
Czech Republic»: ENTHEROtest-16,
STAPHYtest-16, Streptotest-16, En-COCCUStest-
16; for lactobacilli and bifidobacteria
identification - API 50 CHL (bioMerieux).
Bacteria content in 1 g of material was calculated
by the number of microorganism colonies
grown. Results. A pattern of changes of mucous
microflora of the intestine and the state of the
wall of the large intestine of the experimental
animals that had been put under the influence
of anomalous magnetic field is shown. During
evaluation of qualitative and quantitative diversity of microbial community of parietal mucin
of the large intestine of the mice under the influence
of magnetic field on the background and
anomalous levels changes not only in quantity
and frequency of detection of obligate, transitory
flora but also cell elements of mucous
membrane of the wall of the large intestine were
established. Conclusion. The results of the study
allow to make a conclusion about the presence
of reactivity of the parietal microflora of the
intestine of the mice to the influence of the
anomalous magnetic field. This leads to changes
in cell elements in the mucous membrane of
the wall that manifest by infiltration of the connective
tissue stroma by leucocytes and reconstruction
of epithelium, that are features of
dysbiosis.

dysbiosis, magnetic field, intestine microflora, mucous membrane, large intestine