INFLUENCE OF ANTISTAPHYLOCOCCUS ANTIOBIOTIC BATUMIN ON MICROORGANISM BIOFILM FORMATION

Aim. Study the influence of batumin on microorganism
biofilm formation. Materials and
methods. Experimental data on the antimicrobial
effect of batumin on microorganisms and
biofilm formation (BFF) was obtained by
studying 80 strains of bacteria and fungi isolated
from microbial biocenosis of the nose of
staphylococcus carriers and patients during
examination for intestine dysbiosis. 80% pure
batumin was used in the experiments. Antimicrobial
activity of batumin was studied by
serial dilutions method (CLSI, 2005), BFF - by
photometry method (O'Toole G., 2000). The
results were statistically treated by non-parametric
method by using Mann-Whitney criterion.
Results. Mini mal inhibitory concentration
(MIC) of batumin varied from 0.25 mcg/ml to
64 mcg/ml depending on the species of the
studied microorganisms. The most sensitive to
batumin strains were Staphylococcus aureus
when compared with escherichia and klebsiella.
Batumin had no antimicrobial effect on the
studied Candida albicans. Inhibitory effect of
batumin against BFF of staphylococci, klebsiella
and fungi that have an initial level of this
property above 2.5 units was established. While
in strains that have the initial BFF level of 2.5
units or less, batumin stimulated biofilm formation.
Such a dependence was not detected in the
studied Escherichia coli cultures: batumin
stimulated BFF of escherichia in 80 - 90% of
cases. Conclusion. The data obtained uncover
one of the possible mechanisms of microsymbiocenosis
formation in the human associative
symbiosis and open the perspectives for further
studies of batumin not only as an antimicrobial
preparation but also a substance possessing
anti-persistence effect against pathogens.

batumin, biofilm formation, minimal inhibitory concentration, antimicrobial preparations, associative symbiosis