Email this article MOLECULAR-BIOLOGICAL CHARACTERISTICOF YERSINIA ENTEROCOLITICA CIRCULATINGIN VARIOUS REGIONS OF RUSSIANFEDERATION
- Authors: Karimova TV1, Bogumil'chik EA1, Voskresenskaya EA1, Klimov VT1, Tseneva GY.1, Chesnokova MV1, Ivanov LI1, Poutonen TB1, Vasil'eva AV1, Gromova TV1, Karimova TV2, Bogumilchik EA3, Voskresenskaya EA3, Klimov VT4, Tseneva GY.3, Chesnokova MV4, Ivanov LI5, Poutonen TB6, Vasilyeva AV5, Gromova TV5
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Affiliations:
- Center of Hygiene and Epidemiology of Novo -sibirsk Region, Novosibirsk
- Pasteur St. PetersburgResearch Institute of Epidemiology andMicrobiology
- Irkutsk Research Plague ControlInstitute of Siberia and the Far East
- KhabarovskStation for Plague Control
- Center of Hygieneand Epidemiology in the Republic of Karelia,Petrozavodsk, Russia
- Issue: Vol 89, No 1 (2012)
- Pages: 16-21
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13648
- ID: 13648
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Full Text
Abstract
signs and main virulence genes of Yersinia enterocolitica
strains circulating in various regions
of Russian Federation. Materials and methods.
46 strains of Y. enterocolitica of 2 - 4 biotypes
and 401 strains of Y. enterocolitica 1A biotype
isolated in 15 administrative territories of
Russian Federation (Siberian, Far Eastern,
Northwestern, Urals Federal Districts) from
infected people, rodents, agricultural animals,
birds, the environment were studied. Phagotyping
was performed in the reference laboratory
of the Pasteur Institute (Paris). All the Y.
enterocolitica cultures were studied for the presence
of ail, ystB and ystA genes by PCR
method. Presence of virulence plasmid pYV was
determined by gel electrophoresis by T. Kieser
method. Results. 447 strains of Y. enterocolitica
biotype 1A and 2 - 4 were studied. Most of the
strains belonged to serotypes О:3; О:9; О: 5; О:
6,30; О:6,31; О:7,8. Phagotyping was performed
for part of the strains. Phagotypes Xz and Xo
were determined in biotype 1A strains. 2 - 4
biotype strains circulating in Siberia and the Far
East were characterized by phagotype VIII, X3
that are present in other countries, and phagotype
Xz that is spread only in Russia. Phagotypes
IXa, IXb, II that are characteristic for strains
from Canada, South Africa, Japan were not
detected in Russian Federation. All the strains
of 2 - 4 biotypes had ail and ystA genes. Most
of the recently isolated strains had pYV. The
only pathogenicity factor detected in 81.3% of
biotype 1A strains including 14 strains from
patients was ystB gene. These infections were
accompanied by an expressed clinical symptomatology
of enteritis and enterocolitis.
Conclusion. Isolation of 1A biotype strains from
patients necessitates execution of diagnostic
studies of intestinal yersiniosis in patients with
diagnosis «acute intestinal infection of undetermined
etiology».
About the authors
T V Karimova
E A Bogumil'chik
E A Voskresenskaya
V T Klimov
G Ya Tseneva
M V Chesnokova
L I Ivanov
T B Poutonen
A V Vasil'eva
T V Gromova
T V Karimova
Center of Hygiene and Epidemiology of Novo -sibirsk Region, NovosibirskCenter of Hygiene and Epidemiology of Novo -sibirsk Region, Novosibirsk
E A Bogumilchik
Pasteur St. PetersburgResearch Institute of Epidemiology andMicrobiologyPasteur St. PetersburgResearch Institute of Epidemiology andMicrobiology
E A Voskresenskaya
Pasteur St. PetersburgResearch Institute of Epidemiology andMicrobiologyPasteur St. PetersburgResearch Institute of Epidemiology andMicrobiology
V T Klimov
Irkutsk Research Plague ControlInstitute of Siberia and the Far EastIrkutsk Research Plague ControlInstitute of Siberia and the Far East
G Ya Tseneva
Pasteur St. PetersburgResearch Institute of Epidemiology andMicrobiologyPasteur St. PetersburgResearch Institute of Epidemiology andMicrobiology
M V Chesnokova
Irkutsk Research Plague ControlInstitute of Siberia and the Far EastIrkutsk Research Plague ControlInstitute of Siberia and the Far East
L I Ivanov
KhabarovskStation for Plague ControlKhabarovskStation for Plague Control
T B Poutonen
Center of Hygieneand Epidemiology in the Republic of Karelia,Petrozavodsk, RussiaCenter of Hygieneand Epidemiology in the Republic of Karelia,Petrozavodsk, Russia
A V Vasilyeva
KhabarovskStation for Plague ControlKhabarovskStation for Plague Control
T V Gromova
KhabarovskStation for Plague ControlKhabarovskStation for Plague Control
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