IMMUNOCHEMIC ANALYSIS OF RECOMBINANT PROTEIN FlaA OF WESTERN SIBERIAN BORRELIA GARINII ISOLATE


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Abstract

Aim. Study of the ability of Western Siberian Borrelia garinii 20047 isolate recombinant pro- tein FlaA to react with sera antibodies of ixodes tick borreliosis patients. Materials and methods. Recombinant antigen FlaA, sera of ixodes tick borreliosis patients, genetic engineering methods, solid phase EIA, and parametric and nonpara- metric statistical methods were used in the study. Results. Recombinant form of mature flagellar protein FlaA of B.garinii 20047 was obtained. In EIA study of sera of ixodes tick borreliosis patients with migrating erythema and without it, IgM or IgG against FlaA antigen were detected in more than 30% of sera. Indicator of the detection of IgM against FlaA antigen in sera of ixodes tick borrelio- sis patients with migrating erythema and without it was 43.3% and 33.3% respectively. Conclusion. The results obtained show a significant antigenic activity of recombinant protein FlaA of Western Siberian B.garinii isolate and the perspectives of its use for serodiagnostics of ixodes tick borreliosis.

About the authors

V. S Karavaev

Research Institute of Biochemistry, Novosibirsk, Russia

A. V Ryabchenko

Research Institute of Biochemistry, Novosibirsk, Russia

A. B Beklemishev

Research Institute of Biochemistry, Novosibirsk, Russia

References

  1. Горелова Н.Б., Коренберг Э.И., Ковалевский Ю.В. и др. Изоляция боррелий от клещей Ixodes trianguliceps (Ixodidae) и возможное значение этого вида в эпизоотологии иксодовых клещевых боррелиозов. Паразитология. 1996, 30 (1): 13-18.
  2. Масузава Т., Наумов Р.Л., Кудекен М. и др. Обнаружение Borrelia burgdorferi sensu stricto в Московской области, Россия. Мед. паразитол. паразит. бол. 2001, 2: 52.
  3. Мамаев А.Л., Рябченко А.В., Беклемишев А.Б. Исследование зараженности иксодовых клещей, отловленных весной 2008 г. в рекреационной зоне г. Новосибирска, спирохетами Borrelia burgdorferi sensu lato. Бюлл. СО РАМН. 2010, 30 (2): 13-16.
  4. Маниатис Т., Фрич Э., Сэмбрук Д. Молекулярное клонирование. М., 1984.
  5. Рябченко А.В. , Беклемишев А.Б. Мониторинг зараженности клещей Ixodes persulcatus Schulze, населяющих рекреационную зону Новосибирского научного центра, возбудителями Лайм-боррелиоза человека. Вестн. НГУ. 2006, 12 (3-4): 109-110.
  6. Baranton G., De Martino S.J. Borrelia burgdorferi sensu lato diversity and its influence on pathogenicity in humans. Curr. Probl. Dermatol. 2009, 37: 1-17.
  7. Beklemishev A. B., Dobrotvorsky A. K., Piterina A.V. et al. Detection and typing of Borrelia burgdorferi sensu lato genospecies in Ixodes persulcatus ticks in West Siberia, Russia. FEMS Microbiol. Lett. 2003, 227 (2): 157-161.
  8. Ge Y., Charon N. W. FlaA, a putative flagellar outer sheath protein, is not an immunodominant antigen associated with Lyme disease. Infect. Immun. 1997, 65: 2992-2995.
  9. Ge Y., li C., Corum L. et al. Structure and expression of the flaA periplasmic flagellar protein of Borrelia burgdorferi. J. Bacteriol. 1998, 180 (5): 2418-2425.
  10. Gilmore R. D., Murphree J. R. L., James A. M. et al. The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early Lyme disease is the flaA gene product. J. Clin. Microbiol. 1999, 37: 548-552.
  11. Laemmli U.K. Cleavage of structural proteing during the assembly of the head of bacteriophage. Nature. 1970, 227: 680-685.
  12. Panelius J., Lahdenne P., Saxen H. et al. Recombinant flagellin A proteins from borrelia burgdorferi sensu stricto, B.afzelii, and B. garinii in serodiagnosis of lyme borreliosis. J. Clin. Microbiol. 2001, 39 (11): 4013-4019.
  13. Postic D., Korenberg E., Gorelova N. et al. Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incedence of mixed isolates. Res. Microbiol. 1997, 148 (8): 691-702.
  14. Trevejo R.T., Krause P. J., Sikand V. K. et al. Evaluation of two-test serodiagnostic method for early Lyme disease in clinical practice. J. Infect. Dis. 1999, 179: 931-938.

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