Email this article SEROLOGICAL ACTIVITY OF VACCINE AND CIRCULATING BORDETELLA PERTUSSIS STRAINS
- Authors: Zaitsev E.M1, Krasnoproshina L.I1, Mertsalova N.U1, Mazurova I.K2, Zakharova N.S1
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
- Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russia
- Issue: Vol 88, No 2 (2011)
- Pages: 78-82
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13508
- ID: 13508
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Abstract
Aim. To study activity of vaccine and circulating strains of Bordetella pertussis in serological reactions with serum samples from healthy vaccinated children and children with pertussis infection. Materials and methods. One hundred forty-six serum samples from children with pertussis infection as well as 158 samples from healthy vaccinated children aged 3—5 years old were studied. Serologic activity of 3 vaccine strains and 7 strains of B.pertussis isolated from patients with pertussis in 2001—2005 against sera of children with pertussis infection or vaccinated children was assessed with hemagglutination assay (HA), radial gel immunodiffusion (RGI), and immunoelectro- phoresis (IEP). Results. In HA both serum samples of infected and vaccinated children were equally active in agglutination of microbial preparations prepared from vaccine or recently isolated strains of B.pertussis. RGI assay showed that 81 — 84% of serum samples from infected children and 17-19% of samples from healthy vaccinated children reacted with vaccine strains, and 81-85% of samples from infected children and 16-20% of samples from healthy vaccinated children reacted with circulating strains. Sera from patients with pertussis formed identical lines of precipitation with vaccine and circulating strains in RGI assay and three types of precipitation arches profile in IEP. Sera from healthy vaccinated children formed identical precipitation arches with vaccine and circulating strains in RGI assay and one type of precipitation arches profile in IEP. Conclusion. Antibodies of patients with pertussis were equally active against vaccine and circulating strains of B. pertussis. Antibodies of vaccinated children were also equally active against vaccine and circulating strains although revealed more narrow spectrum of antigens compared to children with pertussis infection.
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СЕРОЛОГИЧЕСКАЯ АКТИВНОСТЬ ВАКЦИННЫХ И ЦИРКУЛИРУЮЩИХ ШТАММОВ BORDETELLA PERTUSSIS×
About the authors
E. M Zaitsev
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
L. I Krasnoproshina
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
N. U Mertsalova
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
I. K Mazurova
Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russia
N. S Zakharova
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
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