Email this article INFLUENCE OF MICROBIAL-EPITHELIAL INTERACTIONS ON BIOLOGICAL CHARACTERISTICS OF VAGINAL MICROFLORA
- Authors: Kremleva E.A1, Cherkasov S.V1, Bukharin O.V1
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Affiliations:
- Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
- Issue: Vol 88, No 2 (2011)
- Pages: 53-57
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13478
- ID: 13478
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Abstract
Aim. Evaluation of influence of microbial epithelial interactions on growth properties and antagonistic activity of dominant and associated vaginal microflora. Materials and methods. Growth characteristics and antagonistic activity changes were studied in associated ( Staphylococcu s aureus, Escherichia coli, Corynebacterium spp. ) and dominant ( Lactobacilu s spp. ) microflora during contact with vaginal epitheliocytes and influence of vaginal epitheliocyte secretory products by using the developed technique. Results. Secretory products of vaginal epitheliocytes and vaginal epitheliocytes differentially influence growth factors of vaginal microbiocenosis. Suppression of S.aureus и Е.coli biomass growth based predominately on extracellular secretory products of epitheliocytes was observed. Stimulation of growth properties was prevalent for lactobacilli and corynebacteria and was observed in contact interaction as well as during effect of epitheliocyte extracellular secretory products. Conclusion. The resulting bacterial-epithelial interaction is a differentiating feature between autochthonous and allochthonous microflora due to the creation of selective advantages for dominant microsymbionts by growth properties and antagonistic activity stimulation, and biomass growth suppression of associated symbionts.
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ВЛИЯНИЕ МИКРОБНО-ЭПИТЕЛИАЛЬНОГО ВЗАИМОДЕЙСТВИЯ НА БИОЛОГИЧЕСКИЕ ХАРАКТЕРИСТИКИ ВАГИНАЛЬНОЙ МИКРОФЛОРЫ×
About the authors
E. A Kremleva
Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
S. V Cherkasov
Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
O. V Bukharin
Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
References
- Клаус Д. (ред.). Лимфоциты. Методы. М., Мир, 1990.
- Лакин Р.Ф. Биометрия. М., Высшая школа, 1990.
- Семенов А.В., Сгибнев А.В., Черкасов С.В., Бухарин О.В. Микробная регуляция антагонистической активности бактерий. Бюлл. экспер. биол. мед 2007, 11: 545-548.
- Aoki S.K., Pamma R., Hernday A.D. et. al. Contact-dependent inhibition of growth in Escherichia coli. Science. 2005, 309(5738): 1245-1248.
- Canny G.O., Trifonova R.T., Kindelberger D.W. et. al. Expression and function of bactericidal/permeability-increasing protein in human genital tract epithelial cells. J. Infect. Dis. 2006, 194: 498-502.
- Lee J.H., Del Sorbo L., Khine A.A. et. al. Modulation of bacterial growth by tumor necro- Контактная информация: Кремлева Е.А., sis factor-α in vitro and in vivo. Am. J. Res. Crit. Care Med. 2003, 168: 1462-1470.
- McCormick B.A. (ed.). Bacterial-epithelial cell cross-talk. Molecular mechanisms in pathogen- esis. New York, Cambridge University Press, 2006.
- Petrof E.O., Claud E.C., Sun J. et. al. Bacteria-free solution derived from Lactobacillus plantarum inhibits multiple NF-κB pathways and inhibits proteasome function. Inflamm. Bowel Dis. 2009, 15(10): 1537-1540
- Pivarcsi A., Nagy I., Koreck A. et. al. Microbial compounds induce the expression of pro-in- flammatory cytokines, chemokines and human beta-defensin-2 in vaginal epithelial cells. Microbes Infect. 2005, 7(9-10): 1117-1127
- Wira C. R., Grant-Tschudy K.S., Crane- Godreau M.A. Epithelial cells in the female reproductive tract: a central role as sentinels of immune protection. Am. J. Reprod. Im- munol. 2005, 53(2): 65-76.
- Yu L., Wang L., Chen S. Toll-like receptors, inflammation and tumor in the human female reproductive tract. Ibid. 2009, 62(1): 1-8
- Zeuthen L.H., Fink L.N., Frokiaer H. Epithelial cells prime the immune response to an array of gut-derived commensals towards a tolerogenic phenotype through distinct actions of thymic stromal lymphopoietin and transforming growth factor-beta. Immunology. 2008, 123(2): 197-208.
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