Email this article IMMUNOBIOLOGICAL P ROPE RTIES OF RECOMBINANT ATOXIC FORMS OF THE PSEUDOMONAS AERUGINOSA EXOTOXIN A
- Authors: Isakov M.A1, Soldatenkova A.S1, Kaloshin A.A1, Mikhaylova N.A1
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
- Issue: Vol 88, No 2 (2011)
- Pages: 37-42
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13467
- ID: 13467
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Abstract
Aim. Evaluation of immunobiological prop- erties of recombinant atoxic forms of the Pseudomonas aeruginosa exotoxin. Materials and methods. 3 recombinant atoxic forms of the P.aeruginosa exotoxin A were produced and studied: aTox1, consisting only of exotoxin A domain 1; aTox1,2, consisting of domain 1 and 2 ; and aTox1,2, Δ 3 , consisting of both domain 1 an d 2, and part of domain 3. Results. aTox1,2 and a T ox1,2, Δ 3 had distinctive antigenic properties. F ormulation s based on these recombinant pro- teins were immunogenic and protected animals from exotoxin A in experimental conditions. Conclusion. These results may be used to construct direct-action immunobiological formulations.
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ИММУНОБИ ОЛОГИЧЕСКИЕ СВОЙСТВА РЕКОМБИНАНТНЫХ АТОКСИЧНЫХ ФОРМ ЭКЗОТОКСИНА А PSEUDOMONAS AERUGINOSA×
About the authors
M. A Isakov
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
A. S Soldatenkova
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
A. A Kaloshin
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
N. A Mikhaylova
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
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