GENETIC CHARACTERISTICS OF NEISSERIA MENINGITIDIS STRAINS OBTAINED FROM HEALTHY CARRIERS DURING MENINGOCOCCAL INFECTION OUTBREAKS


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Abstract

Aim. Genetic and antigenic characterization of Neisseria meningitidis strains isolated during meningococcal infection outbreaks from individuals in contact with patients with generalized form of meningococcal infection. Materials and methods. Strains obtained in 2007—2009 in Moscow during examination of individuals that were in contact with patients during meningococcal infection outbreaks were analyzed. Multilocus sequence typing, genetic subtyping and typing of VR fragment (FetA) techniques were used. Results. Data regarding investigated strains were submitted to the database at http://pubmlst.org/ neisseria/. Previously undescribed sequence types were found in 12 strains, sequence-type could not be determined in 2 strains, 2 strains lacked VR fragment (FetA). Serogroup A meningococci had «P1.5-2,10: F3-5» antigenic profile and belonged to ST-75 and ST-3349 sequence-type, these data does not support the emergence of epidemically significant strains in the territory under surveillance. All typed serogroup C strains and 1 serogroup B strain are of «ST-41/44 complex/Lineage 3» clonal complex. Subtypes of serogroup C meningococci strains match subtypes of strains that cause generalized forms of infection, while serogroup B strains isolated from the carriers and strains isolated from the patients had different antigenic profiles. Ungrouppable strains had notably higher level of genetic and antigenic diversity: only 6 of 16 strains (37.5%) could be sequence-typed using earlier data, all these strains are of clonal complex «ST-53 complex» that consists mostly of strains isolated from the carriers. Conclusion. Ratio of meningococci population circulating in Moscow and subpopulation capable of causing generalized form of meningococcal infection (GFMI) is different for meningococci of various serogroups. Ungrouppable strains isolated from the carriers are highly different from strains causing GFMI.

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ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА ШТАММОВ NEISSERIA MENINGITIDIS, ВЫДЕЛЕННЫХ ОТ ЗДОРОВЫХ НОСИТЕЛЕЙ В ОЧАГАХ МЕНИНГОКОККОВОЙ ИНФЕКЦИИ
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About the authors

K. O Mironov

Central Research Institute of Epidemiology, Moscow, Russia

T. A Tagachenkova

Central Research Institute of Epidemiology, Moscow, Russia

I. S Koroleva

Central Research Institute of Epidemiology, Moscow, Russia

A. E Platonov

Central Research Institute of Epidemiology, Moscow, Russia

G. A Shipulin

Central Research Institute of Epidemiology, Moscow, Russia

References

  1. Костюкова Н.Н., Бехало В.А. Менингококковое носительство: загадки и разгадки. Эпидемиол. инфекц. бол. 2010, 1: 30-34.
  2. Миронов К.О., Платонов А.Е., Королева И.С. и др. Генетические субгруппы бактерий Neisseria meningitidis серогруппы А, выделенных от больных генерализованными формами менингококковой инфекции на территории Москвы в 1969-2006 гг. Журн. микробиол. 2008, 1: 7-12.
  3. Миронов К.О., Платонов А.Е., Королева И.С. и др. Мониторинг бактерий вида Neisseria meningitidis на основании последовательностей вариабельных фрагментов поверхностных белков FetA и PorA. Там же. 2009, 3: 23-27.
  4. Миронов К.О., Платонов А.Е., Шипулин Г.А. и др. Генетическое субтипирование Neisseria meningitidis. Эпидемиол. инфекц. бол. 2005, 3: 23-28.
  5. Тагаченкова Т.А., Королева И.С., Миронов К.О. и др. Менингококковое носительство в очагах менингококковой инфекции. Там же. 2009, 4: 6-10.
  6. Achtman M., van der Ende A., Zhu P. et al. Molecular epidemiology of serogroup A meningitis in Moscow, 1969 to 1997. Emerg. Infect. Dis. 2001, 7 (3): 420-427.
  7. Bart A., Dankert J., van der Ende A. Antigenic variation of the class I outer membrane protein in hyperendemic Neisseria meningitidis strains in the Netherlands. Infect. Immun. 1999, 67 (8): 3842-3846.
  8. Claus H., Elias J., Meinhardt C. et al. Deletion of the meningococcal fetA gene used for antigen sequence typing of invasive and commensal isolates from Germany: frequencies and mechanisms. J. Clin. Microbiol. 2007, 45 (9): 2960-2964.
  9. Fox A.J., Taha M.K., Vogel U. Standardized nonculture techniques recommended for European reference laboratories. FEMS Microbiol. Rev. 2007, 31 (1): 84-88.
  10. Hunter P.R., Gaston M.A. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diver- sity. J. Clin. Microbiol. 1988, 26 (11): 2465-2466.
  11. Jolley K.A., Brehony C., Maiden M.C. Molecular typing of meningococci: recommendations for target choice and nomenclature. FEMS Microbiol. Rev. 2007, 31 (1): 89-96.
  12. Sacchi C.T., Lemos A.P., Brandt M.E. et al. Proposed standardization of Neisseria meningitidis PorA variable-region typing nomenclature. Clin. Diagn. Lab. Immunol. 1998, 5 (6): 845-855.
  13. Thompson E.A., Feavers I.M., Maiden M.C. Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component. Microbiology. 2003, 149 (7): 1849-1858.

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Copyright (c) 2011 Mironov K.O., Tagachenkova T.A., Koroleva I.S., Platonov A.E., Shipulin G.A.

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