ESTIMATION OF SPECIES DIVERSITY OF COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED IN HOSPITALS OF RUSSIAN FEDERATION IN 2009 — 2010


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Abstract

Aim . Comparative analysis of species diversity of sample of coagulase-negative staphylococci (CNS) isolated in hospitals of different specializations. Materials and methods . For identification of 102 CNS strains, biochemical systems manufactured by NPO «Diagnostic Systems», VITEK® 2 Compact, and BBL™ Crystal™ as well as sequencing of fragments of tuf and gap genes were used. Results . Greater differentiating capability of genotyping compared with phenotyping methods for species identification of staphylococci was demonstrated. Six CNS species were identified in the sample: S.epidermidis, S.haemolyticus, S.hominis, S.warneri, S.capitis, and S.pasteuri . The largest species diversity was noted for strains from maternity hospitals in Nizhny Novgorod and Kulakov Scientific Center for Obstetrics, Gynecology and Perinatology. Strains isolated from blood of patients in Bakulev Center for Cardiovascular Surgery were represented mostly by S.epidermidis and S.haemolyticus . Differences in species diversity of CNS — causative agents of neonatal conjunctivitis and omphalitis — were observed. Conclusion . Two species of CNS: S.epidermidis and S.haemolyticus pose special threat as nosocomial pathogens both in hospitals for adults and obstetrical facilities. Additionally, in neonatal units it is necessary to control such species as S.warneri, S.capitis, S.pasteuri .

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ОЦЕНКА ВИДОВОГО РАЗНООБРАЗИЯ КОАГУЛАЗООТРИЦАТЕЛЬНЫХ СТАФИЛОКОККОВ, ВЫДЕЛЕННЫХ В СТАЦИОНАРАХ РОССИЙСКОЙ ФЕДЕРАЦИИ В 2009—2010 ГГ
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About the authors

O. L Voronina

Gamaleya Research Institute of Epidemiology and Microbiology

M. S Kunda

Gamaleya Research Institute of Epidemiology and Microbiology

O. A Dmitrenko

Gamaleya Research Institute of Epidemiology and Microbiology

L. A Lyubasovskaya

Kulakov Scientific Center for Obstetrics, Gynecology and Perinatology, Moscow

O. V Kovalishena

State Medical Academy, Nizhny Novgorod

D. A Popov

Bakulev Scientific Center for Cardiovascular Surgery, Moscow, Russia

V. G Lunin

Gamaleya Research Institute of Epidemiology and Microbiology

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Copyright (c) 2011 Voronina O.L., Kunda M.S., Dmitrenko O.A., Lyubasovskaya L.A., Kovalishena O.V., Popov D.A., Lunin V.G.

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