FEATURES OF EPIDEMIOLOGY AND DIAGNOSTICS OF TOXOPLASMOSIS DURING HIV-INFECTION


Cite item

Full Text

Abstract

Aim. Comparative assessment of effectiveness of serologic methods for toxoplasmosis diagnostics in patients with HIV-infection. Materials and methods. Sera and CSF samples from 166 patients with AIDS stage IIIB were tested for antibodies to Toxoplasma gondii by indirect immunofluorescence, ELISA and immunoblotting. Results of serological tests were compared with clinical, pathological data as well as with results of MRI and PCR. Results. Clinical value of IgG detection in blood and CSF by ELISA was shown — high level of antibodies marked reactivation of the invasion. IgG antibodies in CSF were detected only if high levels of IgG were present in the blood. Detection of antigenic determinants with molecular mass 18—20 and 65—70 kDa in immunoblotting was proposed as a criterion of cerebral toxoplasmosis reactivation. Conclusion. Complex laboratory serologic tests along with data obtained by MRI, PCR and microscopy of T.gondii cysts enhances the effectiveness of toxoplasmosis diagnostics. Knowledge of toxoplasmosis reactivation criteria in patients with AIDS will allow to develop the optimal protocol of toxoplasmosis diagnostics and substantiate measures for its prevention.

Full Text

ОСОБЕННОСТИ ЭПИДЕМИОЛОГИИ И ДИАГНОСТИКИ ТОКСОПЛАЗМОЗА ПРИ ВИЧИНФЕКЦИИ
×

About the authors

E. V Gubareva

Gamaleya Research Institute of Epidemiology and Microbiology; Moscow, Russia

D. B Goncharov

Gamaleya Research Institute of Epidemiology and Microbiology; Moscow, Russia

A. B Peregudova

Clinical Hospital for Infectious Diseases No. 2, Moscow, Russia

Yu. G Parkhomenko

Clinical Hospital for Infectious Diseases No. 2, Moscow, Russia

I. M Tishkevich

Clinical Hospital for Infectious Diseases No. 2, Moscow, Russia

O. A Tishkevich

Clinical Hospital for Infectious Diseases No. 2, Moscow, Russia

N. V Kobets

Clinical Hospital for Infectious Diseases No. 2, Moscow, Russia

References

  1. Гончаров Д.Б. Значение персистенции Toxoplasma gondii в клинической патологии человека. Журн. микробиол. 2006, 4: 92-97.
  2. Гончаров Д.Б., Губарева Е.В., Тишкевич И.М. и др. Изучение персистенции низковирулентного штамма Toxoplasma gondii на модели in vivo. Журн. микробиол. 2009, 4: 107-111.
  3. Долгих Т.И., Запарий Н.С., Кадцына Т.В. и др. Эпидемиологический и иммунологический мониторинг токсоплазмоза в Омской области. Мед. паразитол. 2008, 1: 19-22.
  4. Онищенко Г.Г., Беклемишева О.А., Наркевич М.И. О неотложных мерах по борьбе с распространением заболеваний, вызываемых вирусом иммунодефицита человека. Журн. микробиол. 1999, 1: 5-9.
  5. Покровский В.В., Ермак Т.Н., Беляева В.В. и др. ВИЧ-инфекция: клиника, диагностика и лечение. М., ГЕОТАР-МЕД, 2003.
  6. Сергиев В.П. Паразитарные болезни сегодня и завтра. Качество жизни. Медицина. 2005, 8 (1): 10-15.
  7. Тишкевич О.А., Шахгильдян В.И., Пархоменко Ю.Г. Структура летальных исходов и патологическая анатомия у больных ВИЧинфекцией в Москве. Эпидемиол. инфекц. бол. 2004, 4: 42-46.
  8. Bellanger F., Derouin F., Grangeot-Keros L. et al. Incidence and risk factors of toxoplasmosis in a cohort of human immunodeficiency virus-infected patients: 1988-1995. Clin. Infect. Dis. 1999, 28: 575-581.
  9. Bonnet F., Lewden C., May T. et al. Opportunistic infections as causes of death in VIH-infected patients in the HAART era in France. Scand. J. Infect. Dis. 2005, 37 (6 — 7): 482-487.
  10. Cury P.M., Pulido C.F., Furtado V.M. et al. Autopsy findings in AIDS patients from a reference hospital in Brazil: analysis of 92 cases. Pathol. Res. Pract. 2003, 199 (12): 811-814.
  11. Fekkar A., Bodaghi B., Touafek F. et al. Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and realtime PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis. J. Clin. Microbiol. 2008, 46 (6): 1965-1967.
  12. Leport C., Franck J., Chene G. et al. Immunoblot profile as predictor of toxoplasmic encephalitis in patients infected with human immunodeficiency virus. Clin. Diagn. Lab. Immunol. 2001, 8 (3): 579-584.
  13. Luft B.J., Remington J.S. Toxoplasmic encephalitis in AIDS. Clin. Infect. Dis. 1992, 15: 211-222.
  14. Nissapatorn V. Lessons learned about opportunistic infections in southeast Asia. Southeast. Asian J. Trop. Med. Public Health. 2008, 39 (4): 625-641.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2010 Gubareva E.V., Goncharov D.B., Peregudova A.B., Parkhomenko Y.G., Tishkevich I.M., Tishkevich O.A., Kobets N.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies