MOLECULAR GENETIC ANALYSIS OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM ENVIRONMENT AND PATIENTS IN HEALTH CARE FACILITIES


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Abstract

Aim. Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals. Materials and methods. To study the genetic diversity of 36 strains of P.aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2. Results. Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infec- tion. Conclusion. RAPD technique was effective for analysis of P.aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.

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МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЙ АНАЛИЗ ШТАММОВ PSEUDOMONAS AERUGINOSA, ВЫДЕЛЕННЫХ ИЗ ОБЪЕКТОВ ВНЕШНЕЙ СРЕДЫ И ОТ ПАЦИЕНТОВ ЛЕЧЕБНЫХ УЧРЕЖДЕНИЙ
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About the authors

V. A Antonov

Volgograd Research Institute for Plague Control,Russia

V. V Altukhova

Volgograd Research Institute for Plague Control,Russia

S. S Savchenko

Volgograd Research Institute for Plague Control,Russia

G. A Tkachenko

Volgograd Research Institute for Plague Control,Russia

V. S Zamaraev

Volgograd Research Institute for Plague Control,Russia

S. I Zhukova

Volgograd Research Institute for Plague Control,Russia

O. G Kramar

Volgograd State Medical University, Volgograd, Russia

N. L Matveeva

Volgograd State Medical University, Volgograd, Russia

O. V Ostrovsky

Volgograd State Medical University, Volgograd, Russia

G. P Dudchenko

Volgograd State Medical University, Volgograd, Russia

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Copyright (c) 2010 Antonov V.A., Altukhova V.V., Savchenko S.S., Tkachenko G.A., Zamaraev V.S., Zhukova S.I., Kramar O.G., Matveeva N.L., Ostrovsky O.V., Dudchenko G.P.

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