EXPRESSION OF Toll-LIKE RECEPTORS IN SPLEEN AND LYMPHATIC NODES AFTER IMMUNIZATION BY MUCOSAL ROUTES

Aim. To determine level of Toll-like receptors (TLRs) expression in spleen and lymphatic nodes of mice after immunization by mucosal routes. Materials and methods. Mice were immunized with polycomponent vaccine Immunovac ® either by mucosal or subcutaneous route. Expression of TLRs in spleen, respiratory tract-associated lymphatic nodes as well as in small intestine was measured in immunized mice by flow cytomentry method. Results. After immunization of mice by subcutaneous, intranasal and oral routes level of TLRs expression was different. Significant expression of TLR9 and absence of TLR2 expression was noted after non-parenteral methods of immunization. After oral immunization expression of TLRs was identified in gut- and respiratory tract-associated lymphoid tissue as well as in spleen; after intranasal immunization – in respiratory tract-associated lymphoid tissue, and after subcutaneous immunization — in spleen and respiratory tract-associated lymphoid tissue. Conclusion. After oral immunization expression of TLRs was identified in all studied organs, including spleen. Involvement of spleen to this process allows to assume establishment of not only local but also systemic immunity.

mucosal immunization, Toll-like receptors, polycomponent vaccine Immunovac, mice