Abstract
Aim. To study the role lectin galactose-specific receptor of Vibrio cholerae hemolysin in receptor mechanisms of bacterial cells lysis. Materials and methods. Five strains of V.cholerae eltor ctx - Hly + and 5 strains V.cholerae eltor ctx + Hly - isolated from patients and environment as well as 6 strains from Shigella genus, 3 strains of Escherichia coli , 2 strains of V.cholerae eltor , and 1 strain of V.cholerae non O1 were used in the study. Preparation P-11702 obtained from strain of V.cholerae non O1 was used for the study of bactericidal activity of hemolysin. For neutralization of bactericidal effect antihemolytic serum prepared against P-11702 as well as 1% solutions of carbohydrates (galactose, sucrose, glucose, and N-acetyl-D-galactosamine) were used. Results. It was shown that lytic activity of hemolysin against cholera vibrios and indicator cultures for detection of vibriocins was neutralized by galactose as well as hemolysis of erythrocytes was neutralized by specific antihemolytic serum. Sucrose, glucose, and N-acetyl-D-galactosamine did not have effect on bactericidal and hemolytic activity of hemolysin. Conclusion. Protein-carbohydrate receptor binding of galactose- specific lectin has an important role in realization of bactericidal effect of V.cholerae hemolysin.
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