Email this article PHENOTYPIC CHARACTERISTIC OF STAPHYLOCOCCI AND LOCAL IMMUNITY DURING BACTERIAL CARRIAGE
- Authors: Kartashova O.L1,2, Norkina A.S1,2, Chaynikova I.N1,2, Smolyagin A.I1,2
-
Affiliations:
- Institute of Cellular and Intracellular Symbiosis, Orenburg
- Orenburg State Medical Academy, Russia
- Issue: Vol 86, No 4 (2009)
- Pages: 99-103
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13390
- ID: 13390
Cite item
Full Text
Abstract
Aim. To assess prevalence and intensity of antilactoferrin and sIgA-protease activity in strains of staphylococci and to study the state of local immunity in resident and transitory bacterial carriers. Materials and methods. Strains of Staphylococcus aureus and S.epidermidis isolated from 67 healthy persons, who were tested for staphylococcal carriage with subsequent differentiation of types of carriage according to Chistovitch's method, were studied. Antilactoferrin and sIgA-protease activity as well as levels of lactoferrin and sIgA in nasal secretions were determined using fluoroimmunoassay. Results. Higher prevalence as well as intensity of antilactoferrin and sIgA-protease activity and combination of these characteristics was determined for strains of S.aureus from resident carriers compared with strains from transitory carriers. Higher levels of lactoferrin and sIgA were observed in nasal secretions of resident carriers compared with transitory carriers. Conclusion. Studied factors could be the markers of resident type of carriage that should be considered for the development of effective measures for sanation in persons with prolonged bacterial carriage.
Keywords
Full Text
ФЕНОТИПИЧЕСКАЯ ХАРАКТЕРИСТИКА СТАФИЛОКОККОВ И МЕСТНЫЙ ИММУНИТЕТ ПРИ БАКТЕРИОНОСИТЕЛЬСТВЕ×
About the authors
O. L Kartashova
Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia
A. S Norkina
Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia
I. N Chaynikova
Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia
A. I Smolyagin
Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia
References
- Бухарин О.В. Персистенция патогенных бактерий. М., Медицина, 1999.
- Бухарин О.В., Валышев А.В., Чайникова И.Н. и др. Способ определения антилактоферриновой актив- ности микроорганизмов. Патент 2245923. БИ № 4, 2005.
- Бухарин О.В., Чайникова И.Н., Валышев А.В. и др. Способ определения антииммуноглобулиновой активности микроорганизмов. Патент 2236465. БИ № 26, 2004.
- Валышев А.В., Валышева И.В. Роль антилактоферриновой активности бактерий в их персистенции. Журн. микробиол. 2006, 4: 23-25.
- Лакин Г.Ф. Биометрия. М., Высшая школа, 1990.
- Чистович Г.Н. Эпидемиология и профилактика стафилококковых инфекций. Л., Медицина, 1969.
- Тюрин Ю.А. Протеазная активность микрофлоры кишечника при острых кишечных инфекциях у детей. Автореф.дис.канд.мед.наук. Казань, 2003.
- Усвяцов Б.Я., Чернова О.Л., Гербич И.И. и др. Особенности формирования стафилококкового пейзажа слизистой носа у бактерионосителей разных типов. Журн.микробиол.1994, Приложение: 48-51.
- Хаитов Р.М. Физиология иммунной системы. М., ВИНИТИ РАН, 2001.
- Farnaud S., Evans R.W. Lactoferrin a multifunctional protein with antimicrobial properties. Mol. Immunol. 2003, 40 (7): 395-405.
- Lorenzen D.R., Dux F., Wolk U. et al. Immunoglobulin Al protease, an exoenzyme of pathogenic Neisseria, is a potent inducer of proinflammatory cytokines. J. Exp. Med. 1999, 190: 1049—1058.
- Prokesova L., Porwil-Bobr Z., Baran K. et al. Effect of metalloprolteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes. Immunol. Lett. 1991, 27: 225-230.
- Prokesova L., Potuznikova B., Potempa J. et. al. Cleavage of human immunoglobulins by serine proteinase from Staphylococcus aureus. Ibid. 1992, 31: 259-265.
- Rao M.B., Tanksale A.M., Ghatge M.S. et al. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 1998, 62: 597-635.
- Rey J., Garin N., Spertini F. et al. Targeting of secretory IgA to Peyer,s patch dentritic and T cells after transport by intestinal M cells. J. Immunol. 2004, 172 (5): 3026-3033.
Supplementary files
