ROLE OF CHOLERA TOXIN-INDUCED APOPTOSIS IN ALTERATION OF MACROPHAGES IN MICE

Aim. To study the role of active programmed cell death induced by Vibrio cholerae antigens in alteration of peritoneal macrophages of experimental animals. Materials and methods. Apoptosis was assessed by cytofluorometric analysis with propidium iodide using cytofluorometer «Coulter» as well as on characteristic morphological changes of cells in stained histological preparations. Results. Performed experiments carried out by both methods provide evidence that V.cholerae and its antigens (cholera toxin, neuraminidase, chitinase, and lypopolysaccharide) cause apoptosis of mice peritoneal macrophages, which leads to their alteration. Conclusion. Our results demonstrate that programmed cell death of phagocytes is one of the causes of cytotoxic effect of V.cholerae and its antigens. Performed experiments show the role of apoptosis of macrophages in formation of postimmunization immunosuppression after vaccination against cholera.

apoptosis, macrophages, antigens of V.cholerae, cytotoxicity, mice, postimmunization immunodeficiency