EFFECT OF RECOMBINANT HEAT-SHOCK PROTEIN (rHSP70) OF MYCOBACTERIUM TUBERCULOSIS ON IMMUNOGENICITY OF HAEMOPHILUS INFLUENZAE TYPE b CAPSULAR POLYSACCHARIDE


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Abstract

Aim. To study effect of recombinant heat-shock protein (rHSP70) of Mycobacterium tuberculosis on immunogenicity of Haemophilus influenzae type b capsular polysaccharide (CPSHib). Materials and methods. Capsular polysaccharide was obtained by precipitation with cetavlon, antibody titers and Toll-like receptors (TLRs) were detected by enzyme immunoassay and flow cytometry respectively. Results. rHSP70 modified immune response to chemically conjugated and unconjugated CPSHib. rHSP70 enhanced expression of TLRs 2, 4, 9 on mice splenocytes; encreased levels of CD3 +, CD8 +, NK, CD3/NK (NKT) lymphocytes. Levels of CD4 +, CD25 + (markers of early activation of T-helpers) as well as MHC class II molecules were increased that could be appraised as a shift from T-independent to T-dependent immune response. Difference in antibody titers after 2or 3-dose immunization of mice with 5 mcg/dose of CPSHib in mixture or conjugated with rHSP70 was not revealed. Level of antibodies to rHSP70 in serum samples of mice immunized with CPSHib conjugated with rHSP70 was 6.55 — 8.4 times higher compared to unimmunized animals. Antibodies, which have common antigenic epitopes to human organs and tissues, were not detected. Conclusion. rHSP70 modifies immune response to CPSHib.

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ДЕЙСТВИЕ РЕКОМБИНАНТНОГО БЕЛКА ТЕПЛОВОГО ШОКА MYCOBACTERIUM TUBERCULOSIS (rHSP70) НА ИММУНОГЕННУЮ АКТИВНОСТЬ КАПСУЛЬНОГО ПОЛИСАХАРИДА HАEMOPHILUS INFLUENZAE ТИПА b
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About the authors

Yu. S Shevchik

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

O. V Novikova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. K Akhmatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. A Kurbatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

P. G Sveshnikov

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. E Yastrebova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. P Vaneeva

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

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Copyright (c) 2009 Shevchik Y.S., Novikova O.V., Akhmatova N.K., Kurbatova E.A., Sveshnikov P.G., Yastrebova N.E., Vaneeva N.P.

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