EFFECT OF PAENIBACILLUS POLYMYXA LECTIN ON CYTOKINE STATUS OF ANIMALS

Aim. To study effect of lectin L II of Paenibacillus polymyxa 1460 on cytokine status of mice during modeling of infections caused by Staphylococcus, Escherichia and Yersinia . Materials and methods. Lectin LII of P.polymyxa 1460, bacterial cultures Staphylococcus aureus 209-P, Escherichia coli O1, and Yersinia enterocolitica 12 were used. Mice were inoculated intraperitoneally with 0.2 ml of suspension of bacterial culture grown during 24 hours (5,000 m.c./ml). Lectin (0.4 mcg/ml) in dose 0.2 ml was administered 24 h before and 1 h after inoculation. Serum samples from sacrificed animals were obtained 1, 6, and 24 hours after inoculation and concentrations of IL-1, IL-6, and TNF-α were measured in them using optical density values. Results. It was established that level of TNF-α in serum decreased during staphylococcal infection, whereas levels of IL-1 and IL-6 decreased during all modeled infections. Ability for correction of cytokine balance in organism of experimental animals by administration of lectin 24 h before and 1 h after inoculation with bacteria was demonstrated. Conclusion. Presented studies testify to the effect of bacterial lectin on cytokine-production activity of macrophages during phagocytosis of bacteria and infectious process.

P.polymyxa, lectin, cytokine status, mice