DIAGNOSTICS OF GENITAL HERPESVIRUS INFECTION IN WOMEN WITH TUBAL-PERITONEAL INFERTILITY

Aim. Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various par ts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokineand nitroxidergic regulation. Materials and methods. Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used. Results. In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2 — in 46.03%, CMV — in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women. Conclusion. Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.

tubal-peritoneal infertility, herpesvirus infection, cervical canal, endometrium, ovaries, nitric oxide stable end metabolites, cytokines