Email this article INFLUENCE OF MUTATION L,D-CARBOXYPEPTIDASE-CODING GENE ON DYNAMICS OF LISTERIA INFECTION
- Authors: Yurov D.S1,2, Egorova I.Y.1,2, Varfolomeev A.F1,2, Kolbasov D.V1,2, Ermolaeva S.A1,2
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Affiliations:
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow
- All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
- Issue: Vol 86, No 4 (2009)
- Pages: 55-59
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.08.2009
- URL: https://microbiol.crie.ru/jour/article/view/13324
- ID: 13324
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Abstract
Aim. To study the progress of infection caused by mutant Listeria monocytogenes with deletion of lmo0028 gene coding L,D-carboxypeptidase (protein involved in metabolism of peptidoglycan). Materials and methods. Deletion of lmo0028 gene was performed with site-specific mutagenesis method using wild-type strain EGDe. Virulence was studied on mice of BALB/c line. Results. Strain GIMd0028 with deletion of lmo0028 gene did not differ from parent strain on in vitro growth rate, cytotoxicity and production of listeriolysin O and phospholipase PlcA pathogenicity factors. Effective LD 5 0 for wild-type strain EGDe was 1x10 4 CFU/ mouse and 2x10 5 CFU/mouse for intravenous and intraperitoneal inoculation respectively. Deaths of animals were observed after 3 — 7 days. LD 50 for mutant strain GIMd0028 was 1x10 5 CFU/mouse for intravenous inoculation. It was not possible to determine LD 50 for intraperitoneal inoculation, and infection progressed atypically slowly. For example, deaths of animals were observed during 22 days (time of experiment) starting from day 4 when strain GIMd0028 in dose 10 6 CFU/mouse was used for inoculation. In average, death of 1 — 2 mice out of 75 inoculated was observed. Presence of L.monocytogenes was confirmed by its isolation from liver and brain of dead mice. Conclusion. Disruption of function of lmo0028 gene coding L,D-carboxypeptidase protein, which takes part in metabolism of peptidoglycan, changes dynamics of listeriosis.
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ВЛИЯНИЕ МУТАЦИИ В ГЕНЕ, КОДИРУЮЩЕМ L,D-КАРБОКСИПЕПТИДАЗУ, НА ДИНАМИКУ ЛИСТЕРИОЗНОЙ ИНФЕКЦИИ×
About the authors
D. S Yurov
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
I. Yu Egorova
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
A. F Varfolomeev
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
D. V Kolbasov
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
S. A Ermolaeva
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; All-Russian Research Institute of Veterinary Virology and Microbiology, Moscow, Russia
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