Email this article MECHANISMS OF INTERACTION BETWEEN HIV AND MYCOPLASMA ARGININI IN VITRO
- Authors: Miller G.G1, Rakovskaya I.V1, Pokidysheva L.N1, Korablina E.V1, Titova I.V1
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Affiliations:
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
- Issue: Vol 86, No 4 (2009)
- Pages: 49-53
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13311
- ID: 13311
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Abstract
Aim. Until now, the problem of effective therapy of HIV-infection is not resolved due to integrative type of interaction of HIV virus with target cell — T-lymphocyte. The study was aimed on search of method of deletion of HIV DNA-provirus from cell's genome. Materials and methods. Non-pathogenic for humans Mycoplasma arginini was used for coinfection of HIV-infected cells in model systems in vitro . Results. Complex of mechanisms was documented leading to: blocking up to 50 — 60% of extracellular virus (according to titration results), cancel of apoptosis in infected cells stained on Hoechst, formation of defective vif virions, which together with arginine-desaminase of M.arginini arrange permissive conditions for activation of cellular APOBEC3G with subsequent disruption of DNA- provirus and blocking of viral infection. As studies of ultrastructure showed, listed events resulted from direct interaction of HIV with mycoplasma. Conclusion. The elimination of HIV DNA-provirus is possible by co-infection of T-lymphocytes with M.arginini .
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МЕХАНИЗМЫ ВЗАИМОДЕЙСТВИЯ ВИЧ С MYCOPLASMA ARGININI IN VITRO×
About the authors
G. G Miller
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
I. V Rakovskaya
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
L. N Pokidysheva
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
E. V Korablina
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
I. V Titova
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
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