USE OF MATHEMATICAL MODEL FOR DETERMINATION OF VIRUSNEUTRALIZING ACTIVITY OF ANTIBODIES DURING EXPERIMENTAL SHIV-INFECTION


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Abstract

Aim. To study the role of neutralizing antibodies (NA) on the model of SHIV-infection in pig-tailed macaques immunized by different methods. Materials and methods. Titers of NA were determined using TZM-bl cells; titers corresponding to 50% neutralization were determined from serum dilution — relative luminescence graph. We have proposed to use index of integral neutralizing activity, which mathematically expressed as integral of neutralization function in serum dilutions interval from 1 (or first dilution used) to that giving selected titer (we selected 50% neutralization). Results. On the basis of analysis of data on course of SHIV-infection in previously immunized macaques it was concluded that more clear correlation exists between results of infection and integral neutralizing activity of serum compared with NA titers corresponding to 50% neutralization. Conclusion. Proposed method could be useful for analysis of immunization efficacy against HIV infection.

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ИСПОЛЬЗОВАНИЕ МАТЕМАТИЧЕСКОЙ МОДЕЛИ ДЛЯ ОПРЕДЕЛЕНИЯ ВИРУСНЕЙТРАЛИЗУЮЩЕЙ АКТИВНОСТИ АНТИТЕЛ ПРИ ЭКСПЕРИМЕНТАЛЬНОЙ SHIVИНФЕКЦИИ
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About the authors

I. N Klots

Research Institute of Medical Primatology, SochiAdler, Russia

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