INFLUENCE OF SYNTHETIC ANTIOXIDANTS ON PERSISTENCE CHARACTERISTICS OF MICROORGANISMS

Aim. To assess antipersistence characteristics of synthetic antioxidants belonging to class of triterpenoids. Materials and methods. Synthetic antioxidants synthesized in the Institute of Organic Chemistry of Russian Academy of Science and belonging to class of triterpenoids were used in the study. Antioxidant activity of compounds was assessed by amperometric method using analyzer «Tsvet Yauza-AAA-01» («Chimav tomatika Ltd.», Moscow) and expressed in quercetin units (QU). Influence of synthetic antioxidants on persistence characteristics (antilysozyme, anticomplement, and anticarnosine activities) of Klebsiella pneumoniae 278 (Tarasevitch State Institute of Standardization and Control for Immunobiologic Preparations) and Staphylococcus aureus was determined by conventional methods. Results. Antioxidant activity of studied compounds varied from 1.53 QU to 11.82 QU. Compound No. 5, which had maximal antioxidant activity and was characterized by presence of 2 atoms of oxygen and hydroxyl radical in its structure, influenced on expression of persistence characteristics of studied microorganisms most efficiently. Conclusion. Obtained results could be used for the development of new drugs aimed against persisting pathogens.

antioxidants, opportunistic microorganisms, persistence, antioxidant activity