Email this article COMPARATIVE STUDY OF EXPERIMENTAL CONJUGATED AND COMBINED VACCINES AGAINST GROUP B STREPTOCOCCI
- Authors: Ditina M.A1,2, Meringova L.F1,2, Leontyeva G.F1,2, Grabovskaya K.B1,2, Yang A.1,2, Shen Z.1,2, Suvorov A.N1,2
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Affiliations:
- Research Institute of Experimental Medicine, SaintPetersburg, Russia
- Beijing Children’s Hospital, China
- Issue: Vol 86, No 1 (2009)
- Pages: 37-41
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.02.2009
- URL: https://microbiol.crie.ru/jour/article/view/13245
- ID: 13245
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Abstract
Immunogenic properties of recombinant protein ScaAB mixed with polypeptide P6 as well as conjugate of the former with capsular polysaccharide type III (PSIII) were assessed. Protein-polysaccharide conjugate was synthesized by reductive amination method. Immunogenicity was studied on the mouse model using aluminium hydroxide as adjuvant. Antibody titers in antisera were measured by immunoenzyme assay. Functional activity of antibodies was evaluated by opsonophagocytic test. Immunization with ScaAB-PSIII conjugate resulted in increased immune response to both ScaAB and polysaccharide. Administration of ScaAB and P6 proteins mixture compared to their separated administration led to increased of antibody titer and prolonged circulation of specific antibodies. Injection of studied vaccines increased opsonizing activity of antisera compared to immunization with uncombined components.
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СРАВНИТЕЛЬНОЕ ИЗУЧЕНИЕ ЭКСПЕРИМЕНТАЛЬНЫХ КОНЪЮГИРОВАННОЙ И КОМБИНИРОВАННОЙ ВАКЦИН ПРОТИВ СТРЕПТОКОККОВ ГРУППЫ В×
About the authors
M. A Ditina
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
L. F Meringova
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
G. F Leontyeva
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
K. B Grabovskaya
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
A. Yang
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
Z. Shen
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
A. N Suvorov
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Beijing Children’s Hospital, China
References
- Агафонова И.И. Особенности математического описания экспериментальных данных при анализе распределения сывороточных IgG, специфичных в отношении вируса гриппа. Вестн. АМН СССР. 1991, 11: 11-12.
- Булгакова Т.Н., Грабовская К.Б., Тотолян А.А. Стрептококки группы В в патологии человека. Журн. микробиол. 1983, 5: 7-15.
- Мерингова Л.Ф., Леонтьева Г.Ф., Суворов А.Н. Модификация иммуногенности рекомбинантного фрагмента белка Bac стрептококка группы В методом химического связывания с декстраном. Там же. 2007, 3: 22-25.
- Мерингова Л.Ф., Леонтьева Г.Ф., Устинович И.А. и др. Сравнительное изучение антигенных и иммуногенных свойств рекомбинантных полипептидов - фрагментов белка Вас стрептококка группы В. Мед. иммунол. 2004, 6 (6): 493-498.
- Орлова Н.В., Проскурнин М.А., Самбурова В.А., Цветков П.В. Определение полисахаридов и липополисахаридов при помощи спектрофотометрии и термолинзовой спектрометрии. Журн. анал. хим. 2003, 58 (2): 171-173.
- Суворов А.Н., Грабовская К.Б., Леонтьева Г.Ф. и др. Рекомбинан тные вак цины прот ив стрептококков группы В как средство специфической профилактики. Мед. акад. журн. 2006, 1: 13-19.
- Backer C.J., Edwards M.S. Group B streptococcal conjugate vaccines. Arch. Dis. Child. 2003, 88: 375-378.
- Cheng Q., Debol S., Lam H. et al. Immunization with C5a peptidase or peptidase-type iii polysaccharide conjugate vaccines enhances clearance of group B streptococci from lungs of infected mice. Infect. Immun. 2002, 70 (11): 6409-6415.
- Gravekamp C., Kasper D.L., Paoletti L.C. et al. Protein as a carrier for type iii capsular polysacchride and as a protective protein in group B streptococcal vaccines. Ibid. 1999, 67 (5): 2491-2496.
- Heath P.T., Feldman R.G. Vaccination against group B streptococcus. Exp. Rev. Vac. 2005, 4 (5): 207-218.
- Madoff L.C., Paoletti L.C., Tai J.Y., Kasper D.L. Maternal immunization of mice with group B streptococcal type iii polysaccharide-beta C protein conjugate elicits protective antibody to multiple serotypes. J. Clin. Invest. 1994, 94: 286-292.
- Marquest M.B., Kasper D.L., Shroff A. et al. Functional activity of antibodies to the group B polysaccharide of group B streptococci elicited by a polysaccharide-protein conjugate vaccine. Infect. Immun. 1994, 62 (5): 1593-1599.
- Paoletti L.C., Madoff L.C., Kasper D.L. Surface structures of group B streptococcus important in human immunity. In: Gram-positive pathogens. Washington, Asm. Press, 2000, p. 153-161.
- Shen X., Lagergard T., Yang Y. et al. Group B streptococcus capsular polysaccharide-cholera toxin B subunit conjugate vaccines preparared by different methods for intranasal immunization. Infect. Immun. 2001, 69 (1): 1-10.
- Vorobyeva E.I., Meringova L.F., Leontieva G.F. et al. Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogencity. Folia Microbiol. 2005, 50: 172-176.
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