Email this article STUDY OF BIOLOGICAL PROPERTIES OF COLD-ADAPTED REASSORTANT STRAIN OF INFLUENZA VIRUS SUBTYPE H7N3
- Authors: Desheva J.A1,2,3, Smolonogina T.A1,2,3, Sergeeva M.V1,2,3, Rekstin A.R1,2,3, Swayne D.1,2,3, Klimov A.I1,2,3, Rudenko L.G1,2,3
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Affiliations:
- Research Institute of Experimental Medicine, SaintPetersburg, Russia
- Center for Diseases Control and Prevention, Atlanta
- Southeast Poultry Research Laboratory, Athens, USA
- Issue: Vol 86, No 1 (2009)
- Pages: 31-36
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13240
- ID: 13240
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Abstract
For the development of live attenuated influenza vaccine (LAIV) against influenza virus strains with pandemic potential, method of classic genetic reassortment of donor of attenuation A/Leningrad/134/17/57 (H2N2) [Len/17] with avian apathogenic influenza viruses of different subtypes was used. Strain with genome formula 6:2, which contains HA and NA genes from avian apathogenic virus A/wild duck/Netherlands/12/00 (H7N3) [N7N3-wt] and 6 other genes — from Len/17, was studied. Reassortant strain A/17/ wild duck/Netherlands/00/84 (H7N3) [Len17/ H7] exhibited tsand caphenotype specific for cold-adapted strains. Reassortant was identical on antigenic profile to parent avian virus H7N3-wt. Like cold-adapted donor strain Len/17, Len17/H7 was attenuated for chickens, whereas wild-type parent strain was lethal in 60% of birds after its intravenous challenge. Reassortant strain Len17/H7 was attenuated during intranasal inoculation of 6 EID 50to white mice, which was confirmed by absence of its isolation from the lungs, actively reproduced on nasal mucosa and stimulated specific systemic and local antibody response.
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БИОЛОГИЧЕСКИЕ СВОЙСТВА ХОЛОДОАДАПТИРОВАННОГО РЕАССОРТАНТНОГО ШТАММА ВИРУСА ГРИППА ПОДТИПА H7N3×
About the authors
J. A Desheva
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
T. A Smolonogina
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
M. V Sergeeva
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
A. R Rekstin
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
D. Swayne
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
A. I Klimov
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
L. G Rudenko
Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA
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