STUDY OF BIOLOGICAL PROPERTIES OF COLD-ADAPTED REASSORTANT STRAIN OF INFLUENZA VIRUS SUBTYPE H7N3


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Abstract

For the development of live attenuated influenza vaccine (LAIV) against influenza virus strains with pandemic potential, method of classic genetic reassortment of donor of attenuation A/Leningrad/134/17/57 (H2N2) [Len/17] with avian apathogenic influenza viruses of different subtypes was used. Strain with genome formula 6:2, which contains HA and NA genes from avian apathogenic virus A/wild duck/Netherlands/12/00 (H7N3) [N7N3-wt] and 6 other genes — from Len/17, was studied. Reassortant strain A/17/ wild duck/Netherlands/00/84 (H7N3) [Len17/ H7] exhibited tsand caphenotype specific for cold-adapted strains. Reassortant was identical on antigenic profile to parent avian virus H7N3-wt. Like cold-adapted donor strain Len/17, Len17/H7 was attenuated for chickens, whereas wild-type parent strain was lethal in 60% of birds after its intravenous challenge. Reassortant strain Len17/H7 was attenuated during intranasal inoculation of 6 EID 50to white mice, which was confirmed by absence of its isolation from the lungs, actively reproduced on nasal mucosa and stimulated specific systemic and local antibody response.

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БИОЛОГИЧЕСКИЕ СВОЙСТВА ХОЛОДОАДАПТИРОВАННОГО РЕАССОРТАНТНОГО ШТАММА ВИРУСА ГРИППА ПОДТИПА H7N3
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About the authors

J. A Desheva

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

T. A Smolonogina

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

M. V Sergeeva

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

A. R Rekstin

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

D. Swayne

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

A. I Klimov

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

L. G Rudenko

Research Institute of Experimental Medicine, SaintPetersburg, Russia; Center for Diseases Control and Prevention, Atlanta; Southeast Poultry Research Laboratory, Athens, USA

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Copyright (c) 2009 Desheva J.A., Smolonogina T.A., Sergeeva M.V., Rekstin A.R., Swayne D., Klimov A.I., Rudenko L.G.

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