Email this article INTERACTION OF CLOSTRIDIUM DIFFICILEWITH BACTERIAL ASSOCIATIONS OF PARIETAL BIOFILM IN COLON OF MICE
- Authors: Gosteva V.V1, Klitsunova N.V1, Bondarenko V.M1
-
Affiliations:
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
- Issue: Vol 86, No 1 (2009)
- Pages: 3-6
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.02.2009
- URL: https://microbiol.crie.ru/jour/article/view/13229
- ID: 13229
Cite item
Full Text
Abstract
Using electron microscopy, ultrastructural organization of microbial parietal biofilm in colon of immunodeficient mice line B10-hr rhywas studied before and after peroral inoculation with enteropathogenic strain of Clostridium difficile . It was shown that infection leads to dispersion of normal biofilm in various sites and imbalance in natural proportions of different bacterial associations. Also, clear ultrastructural signs of involution of Gram-negative microorganisms were observed. In the remaining areas of the biofilm, density of bacterial population increased. In the same areas massive intrusion of microorganisms in epitheliocytes occurred with their subsequent localization in phagosomes, phagolysosomes and, in some cases, in the cytoplasm of degenerating eukaryotic cells.
Full Text
ВЗАИМОДЕЙСТВИЕ CLOSTRIDIUM DIFFICILE С БАКТЕРИАЛЬНЫМИ СООБЩЕСТВАМИ ПРИСТЕНОЧНОЙ БИОПЛЕНКИ ТОЛСТОЙ КИШКИ МЫШИ×
About the authors
V. V Gosteva
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
N. V Klitsunova
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
V. M Bondarenko
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
References
- Бондаренко В.М. Роль условно-патогенных бактерий кишечника в полиорганной патологии человека. М., 2007.
- Рыбальченко О.В., Бондаренко В.М., Добрица В.П. Атлас ультраструктуры микробиоты кишечника человека. СПб, ИИЦ ВМА, 2008.
- Смирнова Т.А., Диденко Л.В., Андреев А.Л. и др. Электронно-микроскопическое изучение биопленок, образуемых бактериями Burkholderia cepacia. Микробиология. 2008, 77 (1): 63-70.
- Costerton W., Veeh R., Shirtliff M. et al. The application of biofilm science to the study and control of chronic bacterial infections. J.Clin. Invest. 2003, 112 (10):1466 - 1477.
- Gоphna U., Barlev M., Seijffers R. et al. Curli fibers mediate internalization of Escherichia coli by eukaryotic cells. Infect. Immun. 2001, 69 (4): 2659-2665.
- Jonas K., Tomenius H., Kader A. et al. Roles of curli, cellulose and BapA Salmonella biofilm morphology studied by atomic force microscopy. BMC Microbiol. 2007, 7: 70 - 78.
- Lederboer N.A., Jones B.D. Exopolysaccharide sugars contribute to biofilm formation by Salmonella enterica serovar typhimurium on HEp-2 cells and chicken intestinal epithelium. J.Bacteriol. 2005, 187 (9): 3214 - 3226.
- Macfarlane S., Woodmansey E.J., Macfarlane G.T. Colonization of mucus by human intestinal bacteria and establishment of biofilm communities in two-stage continuous culture system. Appl. Environ. Microbiol. 2005, 71 (11): 7481-7492.
- Meyer G.K.A., Neetz A., Brandes G. et al. Clostridium difficile toxins A and B directly stimulate human mast cells. Infect.Immun. 2007, 75 (8): 3868 - 3876.
- Plummer S., Weaver M.A., Harris J.C. et al. Clostridium difficile pilot study: effects of probiotic supplementation on the incidence of Clostridium difficile diarrhea. Intern. Microbiol. 2004, 7: 59 - 62.
- Poxton I.R. Clostridium difficile. J.Med. Microbiol. 2005, 54: 97 - 100.
- Swidsinski A., Weber J., Loening-Baucke V. et al. Special organization and composition of the mucosal flora in patients with inflammatory bowel disease. J.Clin.Microbiol. 2005, 43 (7): 3380 - 3389.
Supplementary files
