ASSOCIATION OF POLYMORPHIC MARKERS IN GENES OF INNATE IMMUNITY IN PATIENTS WITH PERIODONTITIS AND INFLAMMATORY DISEASES OF UPPER RESPIRATORY TRACT

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Abstract

Aim. Search of association of polymorphisms in DEFВ1, IL-10, TNF-a and TLR2 genes with development of chronic generalized periodontitis in representatives of Ural region (Caucasian race). Materials and methods. 142 patients, that were split into 3 groups, took part in the study: a group of patients with periodontitis, a group with frequent inflammatory disease of upper respiratory tract and a comparison group - healthy donors. A study of polymorphic markers was carried out: DEFB1 (-44G/C), DEFB1 (-20A/G), IL-10 (-1082 A/G), TNF-a (-308 G/A), Arg753Gln and Arg677Trp using PCR in real time mode. Results. Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and Arg753Gln and Arg677Trp genes was determined. Significant differences in distribution of genotypes and alleles of genes IL-10 and TNF-a in the group of patients with periodontitis and comparison group were not detected. Conclusion. DEFB1 (-44G/C) polymorphism can be examined as a marker of periodontitis development risk.

About the authors

N. G. Sarkisyan

Ural Federal University; Institute of Immunology and Physiology; Ural Medical University

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

L. V. Gankovskaya

Pirogov Russian National Research Medical University

Email: noemail@neicon.ru
Russian Federation

I. A. Tuzankina

Ural Federal University; Institute of Immunology and Physiology

Email: noemail@neicon.ru
Russian Federation

O. A. Svitich

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

G. I. Ron

Ural Medical University

Email: noemail@neicon.ru
Russian Federation

V. N. Shershnev

Institute of Industrial Ecology

Email: noemail@neicon.ru
Russian Federation

O. N. Kolyadina

Pirogov Russian National Research Medical University

Email: noemail@neicon.ru
Russian Federation

M. A. Dolgikh

Ural Medical University

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2016 Sarkisyan N.G., Gankovskaya L.V., Tuzankina I.A., Svitich O.A., Ron G.I., Shershnev V.N., Kolyadina O.N., Dolgikh M.A.

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