ASSOCIATION OF POLYMORPHIC MARKERS IN GENES OF INNATE IMMUNITY IN PATIENTS WITH PERIODONTITIS AND INFLAMMATORY DISEASES OF UPPER RESPIRATORY TRACT

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Abstract

Aim. Search of association of polymorphisms in DEFВ1, IL-10, TNF-a and TLR2 genes with development of chronic generalized periodontitis in representatives of Ural region (Caucasian race). Materials and methods. 142 patients, that were split into 3 groups, took part in the study: a group of patients with periodontitis, a group with frequent inflammatory disease of upper respiratory tract and a comparison group - healthy donors. A study of polymorphic markers was carried out: DEFB1 (-44G/C), DEFB1 (-20A/G), IL-10 (-1082 A/G), TNF-a (-308 G/A), Arg753Gln and Arg677Trp using PCR in real time mode. Results. Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and Arg753Gln and Arg677Trp genes was determined. Significant differences in distribution of genotypes and alleles of genes IL-10 and TNF-a in the group of patients with periodontitis and comparison group were not detected. Conclusion. DEFB1 (-44G/C) polymorphism can be examined as a marker of periodontitis development risk.

About the authors

N. G. Sarkisyan

Ural Federal University; Institute of Immunology and Physiology; Ural Medical University

Author for correspondence.
Russian Federation

L. V. Gankovskaya

Pirogov Russian National Research Medical University

Russian Federation

I. A. Tuzankina

Ural Federal University; Institute of Immunology and Physiology

Russian Federation

O. A. Svitich

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

G. I. Ron

Ural Medical University

Russian Federation

V. N. Shershnev

Institute of Industrial Ecology

Russian Federation

O. N. Kolyadina

Pirogov Russian National Research Medical University

Russian Federation

M. A. Dolgikh

Ural Medical University

Russian Federation

References

  1. Ганковская О.А. Исследование ассоциации полиморфных маркеров генов TLR2 и TLR9 с преждевременными родами и внутриутробным инфицированием. Медицинская иммунология. 2010, 12 (1-2): 87-94.
  2. Козодаева М.В., Иванова Е.В., Манулов Б.М. Состояние пародонта у больных сахарным диабетом (обзор). Пародонтология. 2011, 1 (58): 3-7.
  3. Мирсаева Ф.З. Экспресс-диагностика заболеваний внутренних органов у больных хроническим генерализованным пародонтитом. Пародонтология. 2013, 3 (68): 55-58.
  4. Ореховой Л.Ю. Заболевания пародонта. М., Поли Медиа Пресс, 2004.
  5. Свитич О.А., Ганковская Л.В., Рахманова И.В. и др. Ассоциация полиморфных маркеров, локализованных в 5’-нетранслируемой области гена DEFB1, с гипертрофией аденоидных вегетаций. Вестник РГМУ. 2012, 3: 59-62.
  6. Al-Zahrani M. S., Kayal R. A., Bissada N. F. Periodontitis and cardiovascular disease: a review of shared risk factors and new findings supporting a causality hypothesis. Quint. Int. 2006, 37 (1): 11-18.
  7. Chapple I. L., Genco R. Diabetes and periodontal diseases: consensus report of the Joint EFP/ AAP Workshop on Periodontitis and Systemic Diseases. J. Periodontol. 2013, 84 (4): 106112.
  8. Gankovskaya L.V., Svitich O.A., Lavrov V.F. et al. Herpes simplex virus type 2 infection during pregnancy is correlated with elevated TLR9 and TNFa expression in cervical cells. Intern. Trends Immunology. 2014, 2: 62-66.
  9. Jazi M.M., Solgi G., Roosta H.A. et al. HLA-DRB and HLA-DQA/HLA-DQB allele and haplotype frequencies in Iranian patients with aggressive periodontitis. J. Periodontal Res. 2013, 48 (4): 533-539.
  10. Karimbux N.Y., Saraiya V.M., Elangovan S. et al. Interleukin-1 gene polymorphisms and chronic periodontitis in adult whites: a systematic review and meta-analysis. J. Periodontol. 2012, 83 (11): 1407-1419.
  11. Luis-Delgado O., Echevarria-Garcia J. J., Berini-Aytes L. et al. Periodontitis as a risk factor in patients with ischemic heart disease. Med. Oral. 2004, 9(2):125-137.
  12. Schroder N.W., Hermann C., Hamann L. et al. High frequency of polymorphism Arg753Gln of the Toll-like receptor-2 gene detected by a novel allele-specific PCR. J. Mol. Med. 2003, 81 (6): 368-372.
  13. Taba M. Jr., Souza S.L., Mariguela V.C. Periodontal disease: a genetic perspective. Braz. Oral. Res. 2012, 26 (l): 32-8.
  14. Tesse R., Cardinale F., Santostasi T. et al. Association of beta-defensin-1 gene polymorphisms with Pseudomonas aeruginosa airway colonization in cystic fibrosis. Genes Immun. 2008, 9 (1): 57-60.

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Copyright (c) 2016 Sarkisyan N.G., Gankovskaya L.V., Tuzankina I.A., Svitich O.A., Ron G.I., Shershnev V.N., Kolyadina O.N., Dolgikh M.A.

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