Email this article FORMATION OF POPULATION MORBIDITY WITH SALMONELLOSIS CAUSED BY SALMONELLA ENTERITIDIS IN REGIONS WITH INCOMPLETE SUPPLY OF LOCAL POULTRY PRODUCTS
- Authors: Shubin F.N.1, Rakov A.V.1, Kuznetsova N.A.1, Yakubich T.V.2, Snetkova I.P.3
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Affiliations:
- Research Institute of Epidemiology and Microbiology
- Centre of Hygiene and Epidemiology in Sakhalin Region
- 3Centre of Hygiene and Epidemiology, in Jewish AR
- Issue: Vol 94, No 1 (2017)
- Pages: 61-67
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/124
- DOI: https://doi.org/10.36233/0372-9311-2017-1-61-67
- ID: 124
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Abstract
Aim. Study plasmid characteristics of S. enteritidis strains in patients and features of epidemiology of the infection in regions with incomplete supply of population with local poultry production. Materials and methods. Plasmid analysis of microbe strains isolated from 382 patients and 8 samples of products was carried out, and significance of plasmid types in population morbidity was evaluated. Identification of salmonella was carried out by conventional methods, plasmid specter - by Kado C.I. and Liu S.T. (1981) method. Results. 98.4% of strains contained virulence plasmid p38, and 80.1% of strains also had small plasmids. Sakhalin strains were divided into 16 plasmid types (D=0.794), and strains from Jewish AO - 10 (D=0.834). Uniformity of strains in patients during infection outbreaks and in transmission factors was detected. Conclusion. Features of salmonellosis in the studied subjects of Russian Federation are determined by higher risk of import of products containing salmonella. Monitoring based on plasmid analysis is an effective base for epidemiologic control.
About the authors
F. N. Shubin
Research Institute of Epidemiology and Microbiology
Author for correspondence.
Russian Federation
A. V. Rakov
Research Institute of Epidemiology and MicrobiologyRussian Federation
N. A. Kuznetsova
Research Institute of Epidemiology and MicrobiologyRussian Federation
T. V. Yakubich
Centre of Hygiene and Epidemiology in Sakhalin RegionRussian Federation
I. P. Snetkova
3Centre of Hygiene and Epidemiology, in Jewish ARRussian Federation
References
- Черкасский Б.А., Рожнова С.Ш., Христюхина О.А., Соловьева Н.К. Современные особенности эпидемического процесса сальмонеллезов в СССР. Журн. микробиол. 1991, 1: 32-36.
- Шубин Ф.Н., Раков А.В., Кузнецова Н.А., Маслов Д.В., Ананьев В.Ю. Структура популяции Salmonella enteritidis в Приморском крае по данным плазмидного анализа. Журн. микробиол. 2006, 3 (Прил.): 28-32.
- Шубин Ф.Н. Зоонозный сальмонеллез в России: основные аспекты проблемы. Эпидемиология и вакцинопрофилактика. 2015, 1: 28-30.
- Braden C.R. Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clin. Infect. Dis. 2006, 43: 512-517.
- Foley S.L., Lynne A.M., Nayak R. Molecular typing methodologies for microbial source tracking and epidemiological investigations of gram-negative bacterial foodborne pathogens. Infect. Genet. Evol. 2009, 9: 430-440.
- Hunter P.R., Gaston M.A. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 1988, 26: 2465-2466.
- Kado C.I., Liu S.T. Rapid procedure for detection and isolation of large and small plasmid. J. Bacteriol. 1981, 145: 1365-1373.
- Ozdemir R.M., Acar S. Plasmid profile and pulsed-field gel electrophoresis analysis of Salmonella enterica isolates from humans in Turkey. PLoS ONE. 2014,9: e95976.
- Pang J.C., Chiu T.H., Chiou C.S. et al. Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan. J. Appl. Microbiol. 2005, 99: 1472-1483.
- Rodrique D.C., Tauxe R.V., Rowe B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol. Infect. 1990, 105: 21-27.
- Us E., Erdem B., Tekeli A. et al. Investigation of Salmonella serotype Enteritidis isolates by plasmid profiles analysis and pulsed-field gel electrophoresis. Microbiol. Bui. 2011, 45: 210-222.
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