STUDY OF COMPOSITION OF PLANT EXTRACTS, POSSESSING ANTIMICROBIAL EFFECT AGAINST VIBRIO CHOLERAE EL TOR, USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

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Abstract

Aim. Study the composition of plant extracts using high-performance liquid chromatography (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor. Materials and methods. Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method. Results. Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V.cholerae El Tor, were determined. Conclusion. The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in extracts, possessing antimicrobial effect against V.cholerae El Tor strains.

About the authors

O. V. Markina

Rostov-on-Don Institute for Plague Control

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

E. V. Maksimenko

Southern Federal University

Email: noemail@neicon.ru
Russian Federation

N. V. Markin

Southern Federal University

Email: noemail@neicon.ru
Russian Federation

N. A. Selyanskaya

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. I. Shelokhovich

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. B. Mazrukho

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

N. I. Borisenko

Southern Federal University

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2016 Markina O.V., Maksimenko E.V., Markin N.V., Selyanskaya N.A., Shelokhovich A.I., Mazrukho A.B., Borisenko N.I.

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