STUDY OF COMPOSITION OF PLANT EXTRACTS, POSSESSING ANTIMICROBIAL EFFECT AGAINST VIBRIO CHOLERAE EL TOR, USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Cover Page


Cite item

Full Text

Abstract

Aim. Study the composition of plant extracts using high-performance liquid chromatography (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor. Materials and methods. Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method. Results. Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V.cholerae El Tor, were determined. Conclusion. The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in extracts, possessing antimicrobial effect against V.cholerae El Tor strains.

About the authors

O. V. Markina

Rostov-on-Don Institute for Plague Control

Author for correspondence.
Email: noemail@neicon.ru
Россия

E. V. Maksimenko

Southern Federal University

Email: noemail@neicon.ru
Россия

N. V. Markin

Southern Federal University

Email: noemail@neicon.ru
Россия

N. A. Selyanskaya

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Россия

A. I. Shelokhovich

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Россия

A. B. Mazrukho

Rostov-on-Don Institute for Plague Control

Email: noemail@neicon.ru
Россия

N. I. Borisenko

Southern Federal University

Email: noemail@neicon.ru
Россия

References

  1. Астафьева О.В. Использование Glicyrriza glabra, Achillea micrantha Willd. и Helichrysum arenarium L. для разработки биопрепаратов с антибактериальными свойствами. Автореф. дис. канд. биол. наук. Ставрополь, 2013.
  2. Гордон А., Форд Р. Спутник химика. М., 1976.
  3. Akinsinde K.A., Olukoya D.K. Vibriocidal activities of some local herbs. J. Diarrhoeal. Dis. Res. 1995, 13 (2): 127-129.
  4. Cernakova M., Kostalova D. Antimicrobial activity of berberine - aconstituent of Mahonia aquifolium. Folia Microbiol. (Praha). 2002, 47 (4): 375-378.
  5. Cowan M.M. Plant products as antimicrobial agents. Clinical. Microbial. Rev. 1999, 12 (4): 564-582.
  6. Kumar P., Selvi S.S., Govindaraju M. et al. In vitro anti-biofilm and anti-bacterial activity of Junceella juncea for its biomedical application. Asian. Pac. J. Trop. Biomed. 2012, 2 (12): 930935.
  7. Mutalib A. A., Ashwell R. N., Kannan R. R. et al. Antimicrobial and selected in vitro enzyme inhibitory effects ofleaf extracts, flavonols and indole alkaloids isolated from croton menyharthii. Molecules. 2013, 18: 12633-12644.
  8. Sanchez E., Garcia S., Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Appl. Environ. Microbial. 2010, 76 (20): 6888-6894.
  9. Yamasaki S., Asakura M., Basu N. S. et al. Inhibition of virulence potential of Vibrio cholerae by natural compounds. Indian. J. Med. Res. 2011, 133 (2): 232-239.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Markina O.V., Maksimenko E.V., Markin N.V., Selyanskaya N.A., Shelokhovich A.I., Mazrukho A.B., Borisenko N.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies