COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

E. A. Chromova; Хромова ЕЛ. ..; L. A. Semochkin; Семочкин И. А.; E. A. Akhmatova; Ахматова Э. А.; V. N. Stolpnikova; Столпникова В. Н.; S. A. Skhodova; Сходова С. А.; E. V. Sorokina; Сорокина Е. В.; N. K. Akhmatova; Ахматова Н. К.; M. P. Kostinov; Костимов М. П.

doi:10.36233/0372-9311-2016-6-61-65

COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

Authors: Chromova E.A.¹, Semochkin L.A.¹, Akhmatova E.A.¹, Stolpnikova V.N.¹, Skhodova S.A.¹, Sorokina E.V.¹, Akhmatova N.K.¹, Kostinov M.P.¹
Affiliations:
1. Mechnikov Research Institute of Vaccines and Sera
Issue: Vol 93, No 6 (2016)
Pages: 61-65
Section: ORIGINAL RESEARCHES
Submitted: 10.04.2019
Published: 28.12.2016
URL: https://microbiol.crie.ru/jour/article/view/106
DOI: https://doi.org/10.36233/0372-9311-2016-6-61-65
ID: 106

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Abstract

Aim. Study subpopulation structure of lymphocytes in healthy individuals under the effect of various influenza vaccines in an in vitro system. Materials and methods. Evaluation of immunephenotype features of PBMCs, activated in vitro by immune-adjuvanted and unadjuvanted vaccines against influenza in healthy individuals, was carried out by using flow cytometry method. Results. Grippol plus vaccine caused a more pronounced stimulating effect compared with subunit and split-vaccines on NK-cells, cells with markers of early activation CD45/CD25, induced the quantity of natural regulatory cells (CD4/CD25/Foxp3), increase of the number of В-cells and reduced the amount of cell types with apoptosis marker CD45/CD95. Conclusion. Immune-adjuvanted vaccine Grippol plus induced formation of effectors of both innate and adaptive immunity and possessed the most powerful potential regarding activation of various types of immune-competent cells compared with unadjuvanted vaccines.

Keywords

immune-adjuvanted and unadjuvanted vaccines against influenza, lymphocyte subpopulation structure

References

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COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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