COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

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Abstract

Aim. Study subpopulation structure of lymphocytes in healthy individuals under the effect of various influenza vaccines in an in vitro system. Materials and methods. Evaluation of immunephenotype features of PBMCs, activated in vitro by immune-adjuvanted and unadjuvanted vaccines against influenza in healthy individuals, was carried out by using flow cytometry method. Results. Grippol plus vaccine caused a more pronounced stimulating effect compared with subunit and split-vaccines on NK-cells, cells with markers of early activation CD45/CD25, induced the quantity of natural regulatory cells (CD4/CD25/Foxp3), increase of the number of В-cells and reduced the amount of cell types with apoptosis marker CD45/CD95. Conclusion. Immune-adjuvanted vaccine Grippol plus induced formation of effectors of both innate and adaptive immunity and possessed the most powerful potential regarding activation of various types of immune-competent cells compared with unadjuvanted vaccines.

About the authors

E. A. Chromova

Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Russian Federation

L. A. Semochkin

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

E. A. Akhmatova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

V. N. Stolpnikova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

S. A. Skhodova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

E. V. Sorokina

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

N. K. Akhmatova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

M. P. Kostinov

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

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Copyright (c) 2016 Chromova E.A., Semochkin L.A., Akhmatova E.A., Stolpnikova V.N., Skhodova S.A., Sorokina E.V., Akhmatova N.K., Kostinov M.P.

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