COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

E. A. Chromova; Хромова ЕЛ. ..; L. A. Semochkin; Семочкин И. А.; E. A. Akhmatova; Ахматова Э. А.; V. N. Stolpnikova; Столпникова В. Н.; S. A. Skhodova; Сходова С. А.; E. V. Sorokina; Сорокина Е. В.; N. K. Akhmatova; Ахматова Н. К.; M. P. Kostinov; Костимов М. П.

doi:10.36233/0372-9311-2016-6-61-65

COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

Authors: Chromova E.A.¹, Semochkin L.A.¹, Akhmatova E.A.¹, Stolpnikova V.N.¹, Skhodova S.A.¹, Sorokina E.V.¹, Akhmatova N.K.¹, Kostinov M.P.¹
Affiliations:
1. Mechnikov Research Institute of Vaccines and Sera
Issue: Vol 93, No 6 (2016)
Pages: 61-65
Section: ORIGINAL RESEARCHES
URL: https://microbiol.crie.ru/jour/article/view/106
DOI: https://doi.org/10.36233/0372-9311-2016-6-61-65
ID: 106

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Abstract

Aim. Study subpopulation structure of lymphocytes in healthy individuals under the effect of various influenza vaccines in an in vitro system. Materials and methods. Evaluation of immunephenotype features of PBMCs, activated in vitro by immune-adjuvanted and unadjuvanted vaccines against influenza in healthy individuals, was carried out by using flow cytometry method. Results. Grippol plus vaccine caused a more pronounced stimulating effect compared with subunit and split-vaccines on NK-cells, cells with markers of early activation CD45/CD25, induced the quantity of natural regulatory cells (CD4/CD25/Foxp3), increase of the number of В-cells and reduced the amount of cell types with apoptosis marker CD45/CD95. Conclusion. Immune-adjuvanted vaccine Grippol plus induced formation of effectors of both innate and adaptive immunity and possessed the most powerful potential regarding activation of various types of immune-competent cells compared with unadjuvanted vaccines.

Keywords

immune-adjuvanted and unadjuvanted vaccines against influenza, lymphocyte subpopulation structure

References

Ахматова Н.К. Роль апоптоза лимфоцитов периферической крови в механизмах развития геморрагической лихорадки с почечным синдромом. Дисс. к.м.н., Уфа, 2003.
Иванова А.С., Пучкова Н.Г., Некрасов А.В. и др. Механизмы адъювантных эффектов полиоксидония. Иммунология гемопоэза. 2015, 13 (2): 30-92.
Казимирко Н.К., Акимова Е.Е., Завацкий В.Ю., Поляков А.С., Татаренко Д.П. Иммунология физиологической беременности. Молодой ученый. 2014, 3 (6): 132-137.
Biron С.А., Nguyen К. В., Pien G. С. et al. Natural killer cells in antiviral defense: function and regulation by innate cytokines. Annu. Rev. Immunol. 1999, 17: 189-220.
Boyum A. Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 1968, 267: 95-99.
De Martino M., Rossi M.E., Azzari C. et al. Viral load and cd69 molecule expression on freshly isolated and cultured mitogen-stimulated lymphocytes of children with perinatal hiv-1 infection. Clin. Exp. Immunol. 1999, 117 (3): 513-516.
French A. R., Yokoyama W. M. Natural killer cells and viral infections. Curr. Opin. Immunol. 2003, 15:45-51.
Schettini 1 J., Mukheijee P. Physiological role of plasmacytoid dendritic cells and their potential use in cancer immunity. Clin Dev. Immunol. 2008; 2008:106321. doi: 10.1155/2008/106321.
Siston A.M. et al. Pandemic 2009 influenza A(H1N1) virus illness among pregnant women in the United States. JAMA. 2010, 303 (15): 1517-1525.

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COMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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