Email this article PROFILES OF CYTOKINES IN MICE DURING IMMUNIZATION WITH ADTP-VACCINE WITH ACELLULAR PERTUSSIS COMPONENT
- Authors: Zaitsev E.M.1, Poddubikov A.V.1, Britsina M.V.1, Ozeretskovskaya M.N.1, Mertsalova N.U.1, Bazhanova I.G.1
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera
- Issue: Vol 93, No 6 (2016)
- Pages: 49-53
- Section: ORIGINAL RESEARCHES
- Submitted: 10.04.2019
- Published: 28.12.2016
- URL: https://microbiol.crie.ru/jour/article/view/104
- DOI: https://doi.org/10.36233/0372-9311-2016-6-49-53
- ID: 104
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Abstract
Aim. Study cytokine status in mice immunized with vaccines containing acellular pertussis component. Materials and methods. Vaccines developed in Mechnikov RIVS - acellular pertussis vaccine (aPV) and adsorbed pertussis-diphtheria-tetanus vaccine (aDTaP), containing a complex of protective antigens of pertussis microbe - were used in the study. Fi (CBAxCsyBle) line mice weighing 12 - 14 g were immunized intraperitoneally 3 times at an interval of 7 days with aPV and aDTaP at human immunization dose (0.5 ml), containing 25 pg of pertussis component. Intact mice were used as a control group. Levels of IFN-y, IL-2, IL-4, IL-5, IL-12 cytokines were determined after each immunization in enzyme immunoassay using commercial test-systems from Cusabio (China). Results. An increase of levels of IFN-y, IL-2, IL-5, IL-12 and lack of stimulation of production of IL-4 was established in dynamics of immune response after administration of aPV and aDTaP vaccines. Conclusion. The data obtained indicate that immunization of mice with aPV and aDTaP vaccines resulted in activation of production of cytokines characteristic for immune response during pertussis infection and immunization with whole-cellular aDTP-vaccines.
About the authors
E. M. Zaitsev
Mechnikov Research Institute of Vaccines and Sera
Author for correspondence.
Email: noemail@neicon.ru
Россия
A. V. Poddubikov
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
M. V. Britsina
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
M. N. Ozeretskovskaya
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
N. U. Mertsalova
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
I. G. Bazhanova
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
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