PROFILES OF CYTOKINES IN MICE DURING IMMUNIZATION WITH ADTP-VACCINE WITH ACELLULAR PERTUSSIS COMPONENT

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Abstract

Aim. Study cytokine status in mice immunized with vaccines containing acellular pertussis component. Materials and methods. Vaccines developed in Mechnikov RIVS - acellular pertussis vaccine (aPV) and adsorbed pertussis-diphtheria-tetanus vaccine (aDTaP), containing a complex of protective antigens of pertussis microbe - were used in the study. Fi (CBAxCsyBle) line mice weighing 12 - 14 g were immunized intraperitoneally 3 times at an interval of 7 days with aPV and aDTaP at human immunization dose (0.5 ml), containing 25 pg of pertussis component. Intact mice were used as a control group. Levels of IFN-y, IL-2, IL-4, IL-5, IL-12 cytokines were determined after each immunization in enzyme immunoassay using commercial test-systems from Cusabio (China). Results. An increase of levels of IFN-y, IL-2, IL-5, IL-12 and lack of stimulation of production of IL-4 was established in dynamics of immune response after administration of aPV and aDTaP vaccines. Conclusion. The data obtained indicate that immunization of mice with aPV and aDTaP vaccines resulted in activation of production of cytokines characteristic for immune response during pertussis infection and immunization with whole-cellular aDTP-vaccines.

About the authors

E. M. Zaitsev

Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

A. V. Poddubikov

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

M. V. Britsina

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

M. N. Ozeretskovskaya

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

N. U. Mertsalova

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

I. G. Bazhanova

Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2016 Zaitsev E.M., Poddubikov A.V., Britsina M.V., Ozeretskovskaya M.N., Mertsalova N.U., Bazhanova I.G.

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