IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

I. I. Korsakova; Корсакова И. И.; V. A. Antonov; Антонов В. А.; N. P. Khrapova; Храпова Н. П.; T. V. Zamarina; Замарина Т. В.; E. V. Pimenova; Пименова Е. В.; E. E. Kim; Ким Е. Э.; L. K. Merinova; Меринова Л. К.; T. V. Senina; Сенина Т. В.; G. A. Tkachenko; Ткаченко Г. А.; S. S. Savchenko; Савченко С. С.; N. P. Ageeva; Агеева Н. П.; E. V. Molchanova; Молчанова Е. В.; Ya. A. Lopasteiskaya; Лопастейская Я. А.; E. V. Prokhvatilova; Прохватилова Е. В.

doi:10.36233/0372-9311-2016-6-25-34

IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

Authors: Korsakova I.I.¹, Antonov V.A.¹, Khrapova N.P.¹, Zamarina T.V.¹, Pimenova E.V.¹, Kim E.E.¹, Merinova L.K.¹, Senina T.V.¹, Tkachenko G.A.¹, Savchenko S.S.¹, Ageeva N.P.¹, Molchanova E.V.¹, Lopasteiskaya Y.A.¹, Prokhvatilova E.V.¹
Affiliations:
1. Volgograd Research Institute for Plague Control
Issue: Vol 93, No 6 (2016)
Pages: 25-34
Section: ORIGINAL RESEARCHES
URL: https://microbiol.crie.ru/jour/article/view/101
DOI: https://doi.org/10.36233/0372-9311-2016-6-25-34
ID: 101

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Abstract

Aim. Determine an optimal set of the most effective methods of identification and intraspecies typing of causative agents of glanders and melioidosis. Materials and methods. Bacteriologic, immunochemical, molecular-genetic methods were used. Results. A possibility to identify collection strains of pathogenic and closely related Burkholderia in semiautomatic systems is studied. Means of detection of informative variable genome segments of the specified microorganisms were developed, methods of their genetic typing were selected. Effectiveness of application of precipitating mAbs for differentiation of Burkholderia was established. Data on diagnostic possibilities of immunoglobulins fluorescing based on monoclonal antibodies of various etiotropic directionality for detection and identification of B. mallei and B. pseudomallei are generalized. Experimental series of amplification test-systems for identification of glanders and melioidosis causative agents in real-time PCR format are created. Conclusion. A number of methods for identification and typing of glanders and melioidosis causative agents is proposed.

Keywords

glanders and melioidosis causative agents, pathogenic Burkholderia genomes, monoclonal antibodies, intra-species typing

References

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IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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