IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

I. I. Korsakova; Корсакова И. И.; V. A. Antonov; Антонов В. А.; N. P. Khrapova; Храпова Н. П.; T. V. Zamarina; Замарина Т. В.; E. V. Pimenova; Пименова Е. В.; E. E. Kim; Ким Е. Э.; L. K. Merinova; Меринова Л. К.; T. V. Senina; Сенина Т. В.; G. A. Tkachenko; Ткаченко Г. А.; S. S. Savchenko; Савченко С. С.; N. P. Ageeva; Агеева Н. П.; E. V. Molchanova; Молчанова Е. В.; Ya. A. Lopasteiskaya; Лопастейская Я. А.; E. V. Prokhvatilova; Прохватилова Е. В.

doi:10.36233/0372-9311-2016-6-25-34

IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

Authors: Korsakova I.I.¹, Antonov V.A.¹, Khrapova N.P.¹, Zamarina T.V.¹, Pimenova E.V.¹, Kim E.E.¹, Merinova L.K.¹, Senina T.V.¹, Tkachenko G.A.¹, Savchenko S.S.¹, Ageeva N.P.¹, Molchanova E.V.¹, Lopasteiskaya Y.A.¹, Prokhvatilova E.V.¹
Affiliations:
1. Volgograd Research Institute for Plague Control
Issue: Vol 93, No 6 (2016)
Pages: 25-34
Section: ORIGINAL RESEARCHES
Submitted: 10.04.2019
Published: 28.12.2016
URL: https://microbiol.crie.ru/jour/article/view/101
DOI: https://doi.org/10.36233/0372-9311-2016-6-25-34
ID: 101

Cite item

Full Text

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

Aim. Determine an optimal set of the most effective methods of identification and intraspecies typing of causative agents of glanders and melioidosis. Materials and methods. Bacteriologic, immunochemical, molecular-genetic methods were used. Results. A possibility to identify collection strains of pathogenic and closely related Burkholderia in semiautomatic systems is studied. Means of detection of informative variable genome segments of the specified microorganisms were developed, methods of their genetic typing were selected. Effectiveness of application of precipitating mAbs for differentiation of Burkholderia was established. Data on diagnostic possibilities of immunoglobulins fluorescing based on monoclonal antibodies of various etiotropic directionality for detection and identification of B. mallei and B. pseudomallei are generalized. Experimental series of amplification test-systems for identification of glanders and melioidosis causative agents in real-time PCR format are created. Conclusion. A number of methods for identification and typing of glanders and melioidosis causative agents is proposed.

Keywords

glanders and melioidosis causative agents, pathogenic Burkholderia genomes, monoclonal antibodies, intra-species typing

References

Жога Л.К., Илюхин В.И., Самыгин В.М. Транспортная среда для патогенных псевдомонад. Клиническая лабораторная диагностика. 1995, 1: 38-40.
Мазин А.В., Кузнеделов К.Д., Краев А.С., Холодилов Н.Г. Методы молекулярной генетики и генной инженерии. Новосибирск, Наука, Сиб. отделение, 1990.
Онищенко Г.Г., Кутырев В.В. Лабораторная диагностика опасных инфекционных болезней. Практическое руководство. М., Шико, 2013.
Прохватилова Е. В., Антонов В.А., Викторов Д. В., Храпова Н. П., Ткаченко Г. А., Илюхин В.И., Захарова И.Б., Гришина М.А., Плеханова Н.Г., Новицкая И.В., Кулаков М.Я., Булатова Т.В., Корсакова И.И., Савченко С.С., Бондарева О.С., Тетерятникова Н.Н., Сенина Т.В., Лопастейская Я.А., Батурин А.А., Куликова А.С. Сравнительная оценка информативности иммунологических и молекулярно-генетических методов и средств на этапах специфической индикации возбудителя мелиоидоза. Клиническая лабораторная диагностика. 2014, 12: 55-59.
Ряпис Л.А., Илюхин В.И., Вострова Е.И., Джузенов А. А. Лабораторная диагностика клинически значимых видов псевдомонад. Лаб. дело. 1988,12: 66-71.
Ряпис Л.А., Тарасова Т.Д. Перспективы применения хромосомной трансформации для идентификации возбудителей мелиоидоза. Журн. микробиол. 1988, 8: 16-19.
Храпова Н. П., Алексеев В. В., Корсакова И. И., Дрефс Н ,М., Ломова Л. В., Булатова Т. В., Напалкова Г.М. Применение сапных и мелиоидозных моноклональных антител различной эпитопной направленности для обнаружения и идентификации патогенных буркхольдерий. Проблемы особо опасных инфекций. 2011, 107: 66-69.
Anuntagool N., Panichakul Т, Aramsri Р. et al. Shedding of lipopolysaccaride and 200-kDa suface antigen during the in vitro growth of virulent Ara- and avirulent Ara+ Burkholderia pseudomallei. Acta tropica. 2000, 74: 221-228.
Duangsonk K., Gal D., Mayo M. et al. Use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of Burkholderia pseudomallei. J. Clin. Microbiol. 2006, 44: 1323-1334.
Goding J.W. Monoclonal antibodies: principles and practice. Acad. Press, 1986.
Kim H.Y., Tsai S., Lo S.C. et al. Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system. PLoS one. 2011, 5: el9867.
Shelly D.B., Spilker X, Gracely E.J. Utility of commercial systems for identification of Burkholderia cepacia complex from cystic fibrosis sputum culture. J. Clin. Microbiol. 2000, 38:3112-3115.
Tomaso H., Scholz H.C., Al Dahouk S. et al. Development of 5-nuclease real-time PCRassays for the rapid identification of the Burkholderia mallei/Burkholderia pseudomallei complex. Diagn. Mol. Pathgl. 2004,13: 247-253.
U'Ren J.M., Schupp J.M., Pearson T. et al. Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotyping. BMC Microbiol. 2007, 1: 7-23.
Vandamme P. Polyphasic taxonomy in practice: the Burkholderia cepacia challenge. WFCC Newsletter. 2002, 34:17-24.

Supplementary files

Supplementary Files

Action

1. JATS XML

Download

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

This website uses cookies