MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY

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Abstract

Aim. Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact30. Materials and methods. Species identification of18 400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bac-teriologic analyzer VITEK2 Compact30 (BioMerieux, France) and MALDI-TOF-MS analysis method on AutoflexIII (Bruker Daltonics, Germany) mass-spectrometer. Results. Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), P-hemolytic staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%). Conclusion. Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed, cost and authenticity of identification of a wide spectrum of microorganisms.

About the authors

T. V. Priputnevich

Scientific Centre of Obstetrics, Gynecology and Perinatology

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

A. R. Melkumyan

Scientific Centre of Obstetrics, Gynecology and Perinatology

Email: noemail@neicon.ru
Russian Federation

L. A. Lyubasovskaya

Scientific Centre of Obstetrics, Gynecology and Perinatology

Email: noemail@neicon.ru
Russian Federation

V. V. Muravieva

Scientific Centre of Obstetrics, Gynecology and Perinatology

Email: noemail@neicon.ru
Russian Federation

E. N. Ilina

Research Institute of Physical-Chemical Medicine

Email: noemail@neicon.ru
Russian Federation

G. T. Sukhikh

Scientific Centre of Obstetrics, Gynecology and Perinatology

Email: noemail@neicon.ru
Russian Federation

References

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Copyright (c) 2016 Priputnevich T.V., Melkumyan A.R., Lyubasovskaya L.A., Muravieva V.V., Ilina E.N., Sukhikh G.T.

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