MOLECULAR EPIDEMIOLOGY OF HEPATITIS C IN CENTERS OF HEMODIALYSIS IN ST. PETERSBURG


Cite item

Full Text

Abstract

Aim. Study molecular epidemiology of hepatitis C (HC) in 5 departments of hemodialysis (DH) in St. Petersburg. Materials and methods. Sequences of nucleotides of 93 isolates including 67 isolates from patents of 5 DH and 26 isolates from patients, who never had hemodialysis in anamnesis, were obtained in 2010 by a method of limited sequencing of NS5B region of HC virus genome. Phyologenetic analysis was carried out by using PHYLIP version 3.69 program package. Evolution differences were evaluated in DNADIST program using F84 algorithm. Phylogenetic trees were constructed by using nearest neighbor and UOGMA methods in PHYLIP program package. Results. Subtype lb was established to dominate in all the DH (69.2 - 92.9%) and the same isolates of HC virus were detected in DH, that were isolated for the first time in 1999. Comparatively higher proportion of isolates of HC subgenotype 3a (26.7 - 30.8%) was detected in 2 of 5 DH in 2010. The same proportion of a isolates was detected in the control group. Conclusion. The fact that HC 3a virus isolates were detected in DH in a higher proportion is proof that they have successfully integrated into circulation among dialysis patients over the last decade.

About the authors

S. L Mukomolov

Pasteur Research Institute of Epidemio1ogy and Microbio1ogy, St. Petersburg, Russia; St. Petersburg State University, Russia

T. Tallo

Agency of Pub1ic Hea1thcare of Sweden, Stockho1m, Sweden; Nationa1 Institute of Hea1th Deve1opment, Ta11inn, Estonia

E. V Sinaiskaya

Pasteur Research Institute of Epidemio1ogy and Microbio1ogy, St. Petersburg, Russia

P. N Kisly

Pasteur Research Institute of Epidemio1ogy and Microbio1ogy, St. Petersburg, Russia

G. F Trifonova

Pasteur Research Institute of Epidemio1ogy and Microbio1ogy, St. Petersburg, Russia

V. V Gerasimova

Pasteur Research Institute of Epidemio1ogy and Microbio1ogy, St. Petersburg, Russia

H. Norder

Gothenburg University, Gothenburg, Sweden

References

  1. Зубкин М.Л., Селиванов Н.А., Стаханова В.М., Новоженов В.Г., Селькова Е.П., Кучерова Т.Е., Баранова Ф.С., Кожокарь Ю.В., Киркман В.В., Касапова Е.Н., Стенина И.И., Львов Д.К. Распространенность и особенности вирусных гепатитов В и С в условиях лечения гемодиализом. Вопр. вирусол. 2000, 1: 10-14.
  2. Мукомолов С.Л., Шляхтенко Л.И., Левакова И.А., Калинина О.В. В: Вирусные гепатиты в Российской Федерации. СПб, НИИЭМ им. Пастера, 2000.
  3. Мукомолов С.Л., Левакова И.А., Синайская Е.В., Сулягина Л.В., Кислый П.Н., Тимаховская Г.Ю., Иванова Н.В., Старосельский К.Г., Ряснянский В.Ю. Эпидемиологическая характеристика вирусных гепатитов В и С в отделениях гемодиализа в Санкт-Петербурге в современный период. Регистрация гемоконтактных вирусных гепатитов и серологические маркеры вирусных гепатитов В и С у пациентов отделений гемодиализа. Инфекция и иммунитет. 2011, 1 (2): 143-150.
  4. Мукомолов С.Л., Левакова И.А. Эпидемиологическая характеристика хронических вирусных гепатитов в Российской Федерации. Инфекция и иммунитет. 2011, 1 (3): 255262.
  5. Amroth G., Ekermo B., Akerlind B. et al. Monitoring hepatitis C infection in a major Swedish nephrology unit and molecular resolution of a new case of nosocomial transmission. J. Med. Virol. 2010, 82 (2): 249-256.
  6. Choo Q.-L., Kuo G., Weiner A.J. et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989, 244 (4902): 359-362.
  7. Choo Q.-L., Richman K., Han J.H. et al. Genetic organization and diversity of the Hepatitis C virus. Proc. Natl. Acad. Sci. USA. 1991, 88: 2451-2455.
  8. Furusyo N., Kubo N., Nakashima H. et al. Confirmation of nosocomial hepatitis C virus infection in a hemodialysis unit. Infect. Control. Hosp. Epidemiol. 2004, 25: 584-590.
  9. Gutelius B., Perz J.F., Parker M.M. et al. Multiple clusters of hepatitis virus infections associated with anesthesia for outpatient endoscopy procedures. Gastroenterology. 2010, 139: 163170.
  10. Hmaied F., Ben Mamou M., Dubois M. et al. Determining the source of nosocomial transmission in hemodialysis units in Tunisia by sequencing NS5b and E2 sequences of HCV. J. Med. Virol. 2007,79: 1089-1094.
  11. Hosseini-Moghaddam S.M., Keyvani H., Kasiri H. et al. Distribution of Hepatitis C virus genotypes among hemodialysis patients in Tehran - a multicenter study. J. Med. Virol. 2006,78: 569-573.
  12. Jadoul M., Poignet J.L., Geddes C. et al. The changing epidemiology of Hepatitis C virus infection in hemodialysis: European multicentre study. Nephrol. Dial. Transplant. 2004, 19: 904-909.
  13. Johnson D.W., Dent H., Yao Q. et al. Frequencies of Hepatitis B and C infection among hemodialysis and peritoneal dialysis patients in Asia-Pacific countries: analysis of registry data. Nephrol. Dial. Transplant. 2009, 24: 1598-1603.
  14. Kalinina O., Norder H., fetrov T., Zhdanov K., Barzunova M., Plotnikova V., Mukomolov S., Magnius L.O. Shift in predominating subtype of HCV from 1b to 3a in St.Petersburg mediated by increase in injecting drug use. J. Med. Virol. 2001, 65: 517-524.
  15. Norder H., Bergstrom A., Uhnoo I. et al. Confirmation of nosocomial transmission of hepatitis C virus by phylogenetic analysis of the NS5B region. J. Clin. Microbiol. 1998, 36: 30663069.
  16. Paintsil E., Verovochkin S., Dukhovlinova E. et al. Hepatitis C virus infection among drug injectors in St.petersburg, Russia: social and molecular epidemiology of an endemic infection. Addiction. 2009, 104: 1881-1890.
  17. Panella H., Rius C., Cayla J.A. Transmission of hepatitis C virus during computed tomography scanning with contrast. Emerg. Infect. Dis. 2008,14: 333-335
  18. Ross R.S., Viazov S., Clauberg R. et al. Lack of de novo hepatitis C virus infections and absence of nosocomial transmission of GB virus C in a large cohort of German haemodialysis patients. J. Viral. Hepat. 2009, 16 (4): 230-238.
  19. Simmonds P., Bukh J., Combet C. et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology. 2005, 42: 962-973.
  20. Tallo T., Norder H., Tefanova V. et al. Genetic characterization of hepatitis C virus strains in Estonia: fluctuations in the predominating subtype with time. J. Med. Virol. 2007, 79 (4): 374382.
  21. Thompson N.D., Hellinger WC., Kay R.S. et al. Healthcare-associated hepatitis C virus transmission among patients in an abdominal organ transplant center. Transpl. Infect. Dis. 2009, 11: 324-329.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2014 Mukomolov S.L., Tallo T., Sinaiskaya E.V., Kisly P.N., Trifonova G.F., Gerasimova V.V., Norder H.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies