FEATURES OF POPULATION COMPOSITION OF PERIPHERAL BLOOD CXCR3-POSITIVE LYMPHOCYTES IN CHRONIC VIRAL HEPATITIS C PATIENTS


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Abstract

Aim. Quantitative determination of CXCR3+, CCR5+ and CCR6+ cells in major lymphocyte populations: T-helpers (Th), cytotoxic T-lymphocytes (CTL), natural killers (NK) and T-natural killer cells (TNK), B-lymphocytes in patients with chronic viral hepatitis C (CVHC). Materials and methods. Content of lymphocyte populations carrying chemokine receptor CXCR3 was studied, chemokine receptors CCR5 and CCR6 were evaluated on T-lymphocytes, in peripheral blood of 19 CVHC patients and 32 conditionally healthy donors. Cell populations were determined by flow cytofluorometry by using various combinations of monoclonal antibodies: for evaluation of Th and CTL (CD3/CD4/CD8/CXCR3/CCR5/CCR6); NK and TNK (CD16/CD56/CD3/ CXCR3); B-cells (CD19/CD45/CXCR3). Results. In patients with CVHV compared with healthy donors a significant increase of quantity of CXCR3-positive Th was detected, however the content of CXCR3-positive CTL did not differ in the groups compared; CXCR3+ NK cell content was lower with equal content of CXCR3+ TNK. Analysis of quantity of CXCR3+ B-cells showed an increase of more than 3.5 times in CVHC patients. Significant differences in relative content of Th and CTL carrying CCR5 and CCR6 were not detected despite a non-significant increase of quantity of CCR5+ and CCR6+ Th. Conclusion. Content of major lymphocyte populations carrying chemokine receptor CXCR3 changed significantly compared with conditionally healthy donors in peripheral blood of CVHC patients. The increase of quantity of CXCR3-positive B-cells may be associated with infection of these cells by HCV or development of extra-liver manifestations of HVHC.

About the authors

A. V Semenov

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

N. A Arsentieva

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

D. S Elezov

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

I. V Kudryavtsev

Research Institute of Experimental Medicine, St. Petersburg, Russia

E. V Esaulenko

State Pediatric Medical University, St. Petersburg, Russia

A. A Totolyan

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

References

  1. Арсентьева Н.А., Семенов А.В., Тотолян Арег А. Роль полиморфизма генов цитокинов при вирусном гепатите С. Инфекция и иммунитет. 2012, 2 (4): 687-698.
  2. Жданов К.В., Гусев Д.А., Чирский В.С. и др. Экспрессия хемокинов и их рецепторов в крови и ткани печени при хроническом вирусном гепатите С. Мед. иммунология. 2007, 9 (4-5): 379-388.
  3. Михайлов М.И. Вирусы гепатита. Клиническая гепатология. 2009, 1: 15-24.
  4. Семенов А.В., Вашукова С.С., Рахманова А.Г. Предварительные итоги лабораторной диагностики вирусных гепатитов В и С в рамках приоритетной национальной программы «Здоровье» в Санкт-Петербурге. Медико-биологические и социально-психологические проблемы безопасности в чрезвычайных ситуациях. 2010, 3: 61-64.
  5. Ahmad A., Alvarez A. Role of NK and NKT cells in the immunopathogenesis of HCV-induced hepatitis. J. Leukoc. Biol. 2004, 76 (4): 743-759.
  6. Apolinario A., Diago M., Lo Iacono O. et al. Increased circulating and intrahepatic T-cell-specific chemokines in chronic hepatitis C: relationship with the type of virological response to peginter-feron plus ribavirin combination therapy. Aliment. Pharmacol. Ther. 2004, 19: 551-562.
  7. Bertoletti A., Maini M.K. Protection or damage: a dual role for the virus-specific cytotoxic T lymphocyte response in hepatitis B and C infection? Curr. Opin. Immunol. 2000, 12: 403-408.
  8. Casrouge A., DecalfJ., Ahloulay M. et al. Evidence for an antagonist form ofthe chemokine CXCL10 in patients chronically infected with HCV J. Clin. Invest. 2011, 121 (1): 308-317.
  9. Khakoo S.I., Thio C.L., Martin M.P. et al. HLA and NK cell inhibitory receptor genes in resolving hepatitis C virus infection. Science. 2004, 305: 872-874.
  10. Larrubia J.R., Benito-Martinez S., Calvino M. et al. Role of chemokines and their receptors in viral persistence and liver damage during chronic hepatitis C virus infection. World J. Gastroenterol. 2008, 14: 7149-7159.
  11. Mizuochi T., Ito M., Saito K., Kasai M. et al. Possible recruitment of peripheral blood CXCR3+ CD27+ CD19+ B cells to the liver of chronic hepatitis C patients. J. Interf. Cytok. Res. 2010, 30 (4): 243-251.
  12. Oo YH., Shetty S., Adams D.H. The role of chemokines in the recruitment of lymphocytes to the liver. Immunol. Liver Dis. 2010, 28: 31-44.
  13. Penna A., Pilli M., Zerbini A. Molecular basis for the defective cytotoxic response in chronic HCV infection. J. Hepatol. 2005, 42 (2): 164-165.
  14. Shimizu Y, Murata H., Kashii Y. et al. CC-chemokine receptor 6 and its ligand macrophage inflammatory protein 3 might be involved in the amplification of local necroinflammatory response in the liver. Hepatology. 2001, 34 (2): 311-319.
  15. Sun J.C., Beilke J.N., Lanier L. Adaptive immune features of natural killer cells. Nature (Cr. Brit.). 2009, 457 (7229): 557-561.
  16. Wozniakowska-Gesicka T. Winiewska-Ligier M., Kaluzynski A. Intrahepatic and peripheral blood NK cells in patients with chronic hepatitis C. Med. Wieku. Rozwoj. 2002, 6 (3): 203-211.
  17. Zeremski M., Petrovic L.M., Chiriboga L. et al. Intrahepatic levels of CXCR3-associated chemok-ines correlate with liver inflammation and fibrosis in chronic hepatitis C. Hepatology. 2008, 48 (5): 1440-50.

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Copyright (c) 2013 Semenov A.V., Arsentieva N.A., Elezov D.S., Kudryavtsev I.V., Esaulenko E.V., Totolyan A.A.

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