MORPHOFUNCTIONAL EVALUATION OF THE STATE OF CELLS OF APUD-SYSTEM BIOMODEL DURING ANTI-PLAGUE VACCINE PROCESS

Aim. Study reaction of cells of APUD-system of lymphoid organs and intestine of guinea pigs at the stages of morpho- and immunogenesis in response to administration of Yersinia pestis EV Research Institute of Epidemiology and Hygiene (RIEH) line vaccine strain. Materials and methods. Vaccine process was modeled in guinea pigs during subcutaneous infection of animals with culture of Yersinia pestis EV RIEH line vaccine strain. Pieces of thymus, spleen, lymphatic nodes and an area of duodenum were taken for histological study. The preparations were stained by solution of hematoxylin and eosin, impregnated with silver by Grimelius and Masson in Hamperl modification. Results. Dose-dependent character of effect of anti-plague vaccination on reaction of apudocytes in immunocompetent organs and duodenum biomodels was established, that indirectly reflects the direction of processes of immunogenesis in central and peripheral organs of immune system. Changes in quantity and morphofunctional state of apudocytes in the intestine of immunized guinea pigs gives evidence of the interest of this segment of APUD-system in the processes of immunogenesis during plague. Conclusion. Range of changes of quantity and functional state of apudocytes in lymphoid organs and intestine of animals during anti-plague vaccination was established. Quantitative parameters of morphofunctional state of intestine adipocytes that may be used for characterization of the intensity of adaptation-compensatory process in the organism of biomodels during modeling of any experimental vaccine process as well as an additional indirect indicator during evaluation of reactogenicity of live anti-plague vaccines were determined.

APUD-system, lymphoid organs, intestine, vaccination