Email this article NEUTROPHIL DEPENDENT BREAKDOWN OFBIOFILMS FORMED BY STAPHYLOCOCCUSAUREUS
- Authors: Chebotar' IV1, Konchakova ED1, Evteeva NI1, Chebotar IV2, Konchakova ED2, Evteeva NI2
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Affiliations:
- State Medical Academy, Nizhny Novgorod,Russia
- Issue: Vol 89, No 1 (2012)
- Pages: 10-15
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.03.2012
- URL: https://microbiol.crie.ru/jour/article/view/13646
- ID: 13646
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Full Text
Abstract
neutrophils with Staphylococcus aureus biofilms
in vitro. Materials and methods. 2 day biofilms
(BF) based on Staphylococcus aureus (strain
5983) were used as a model. Destruction of BF
was evaluated at 5, 15, 30, 45 and 60 minutes of
incubation with neutrophils. Quantitative
evaluation of staphylococci was performed by
using microfilming (Shangrao Optical Instruments)
and a computer program described by
I.V.Chebotar et al., 2010. Measurement of thickness
of BF during reaction with neutrophils was
evaluated by using laser scanning microscope
LSM 510 Meta (Zeiss). Results. Human neutrophils
can influence the breakdown effect on
BF formed by S. auerus. A pronounced breakdown
was observed at 15 minutes of the reaction,
by 45 minutes staphylococci content in the BF
remained only around 2%. Breakdown of BF
was accompanied by an increase of quantity of
viable staphylococci in the supernatant. Around
80% of neutrophils perished at 30 minutes of
interaction with BF. Neutrophil lysate at 30
minutes caused 30% destruction of staphylococci
BF. Conclusion. Adhesive staphylococci are
more resistant to the effect of neutrophils than
BF. Neutrophil dependent destruction of staphylococci
BF could be the result of breakdown
of intracellular matrix by phagocyte secretion
products and direct phagocytosis.
About the authors
I V Chebotar'
E D Konchakova
N I Evteeva
I V Chebotar
State Medical Academy, Nizhny Novgorod,RussiaState Medical Academy, Nizhny Novgorod,Russia
E D Konchakova
State Medical Academy, Nizhny Novgorod,RussiaState Medical Academy, Nizhny Novgorod,Russia
N I Evteeva
State Medical Academy, Nizhny Novgorod,RussiaState Medical Academy, Nizhny Novgorod,Russia
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